Polypeptide with cell-spreading activity

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530395, C07K 1300

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active

051360233

ABSTRACT:
A polypeptide having the cell-spreading activity of human fibronectin. Methods of preparing the polypeptide are described.

REFERENCES:
Kornblihit et al. Embo J. 4(7):1755-1759 (1985).
K. Sekiguchi & S. Hakomori, "Functional Domain Structure of Fibronectin", Proc. Natl. Acad. Sci, U.S.A., 77, 2661-2665, May 1980.
M. D. Pierschbacher, E. G. Hayman & E. Ruoslahti, "Location of the Cell-Attachement Site in Fibronectin with Monoclonal Antibodies and Proteolytic Fragments of the Molecule", Cell, 26, 259-267, Oct. 1981.
M. D. Pierschbacher & E. Ruoslahti, "The Cell Attachment Domain of Fibronectin. Determinaton of the Primary Structure", J. Biol. Chem., 257, 9593-9597, Aug. 1982.
M. D. Pierschbacher, E. G. Hayman & E Ruoslahti, "Synthetic Peptide with Cell Attachment Acitvity of Fibronection", Proc. Natl. Acad. U.S.A., 80, 1224-1227, Mar. 1983.
M. D. Pierschbacher & E. Ruoslahti, "Cell Attachment Activity of Fibronectin Can be Duplicated by Small Synthetic Fragments of the Molecule", Nature, 309, 30-33, May 1984.
A. R. Kornblihtt, K. Vibe-Pedersen & F. E. Baralle, "Human Fibronectin: Cell Specific Alternative mRNA Splicing Generates Polypeptide Chains Differing in the Number of Internal Repeats", Nucl. Acids Res., 12, 5853-5868, Jul. 1984.
M. D. Pierschbacher & E. Ruoslahti, "Variants of the Cell Recognition Site of Fibronectin thaat Retain Attachment-Promoting Activity", Proc. Natl. Acad. Sci. U.S.A., 81, 5985-5988, Oct. 1984.
A. R. Kornblihtt, K. Umezawa, K. Vibe-Pedersen & F. D. Baralle, "Primary Structure of Human Fibronectin: Differential Splicing May Generate at Least 10 Polypeptides from a Single Gene", EMBO J., 4, 1755-1759, 1985.
S. K. Akiyama, E. Hasegawa, T. Hasegawa & K. M. Yamada, "The Interaction of Fibronectin fragments with Fibroblascit Cells", J. Biol. Chem, 260, 13256-13260, Oct. 1985.
M. Obara, M. S. Kang, S. Rocher-Dufour, A. Kornblihtt, J. P. Thiery & K. M. Yamada, "Expression of the Cell-binding Domain of Human Fibronectin in E. coli", FEBS Lett., 213, 261-264, Mar. 1987.
Journal of Biological Chemistry, vol. 258, No. 5, Mar. 30th 1983, pp. 3332-3340, Wachington, DC US; M. Hayashi and K. M. Yamada: "Doman structure of the carboxyl-terminal half of human plasma fibronectin".
Journal of Biological Chemistry, vol. 260, No. 24, Oct. 26th 1986, pp. 13256-13260, Washington, DC, US; S. Akiyama et al.: "The interaction of fibronectin fragments with fibroblastic cells".

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