Polypeptide purification reagents and methods for their use

Details Polypeptide purification reagents and methods for their use Polypeptide purification reagents and methods for their use

2006-08-01

2006-08-01

Weber, Jon (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C530S350000, C530S412000

Reexamination Certificate

active

07083948

ABSTRACT:
The present invention provides polypeptide purification reagents that are, themselves, proteins and methods for using these to purify almost any protein of interest. A polypeptide purification reagent comprises a polypeptide domain that can bind to a protein of interest and a polypeptide domain that confers a propensity to precipitate under conditions where the vast majority of proteins will not precipitate. In addition or as an alternative, a polypeptide purification reagent can comprise amino acid sequences that confer an ability to partition into a phase in a liquid—liquid phase system that the majority of proteins do not partition into. Methods for purifying a protein of interest using such polypeptide purification reagents are also provided. The methods include forming a precipitate comprising the polypeptide purification reagent and the protein of interest under conditions in which the vast majority of proteins do not precipitate and in which the biological activities of both proteins are substantially preserved. The methods further include an optional repurification by one or more reprecipitation steps or by affinity chromatography. In addition or as an alternative, the methods include recovering the protein of interest bound to the polypeptide purification reagent in a phase of a liquid—liquid phase system. The methods further include separating the protein of interest from the polypeptide purification reagent.

REFERENCES:
patent: 4609707 (1986-09-01), Nowinski et al.
patent: 5998588 (1999-12-01), Hoffman et al.
patent: 6207446 (2001-03-01), Szostak et al.
patent: 6258275 (2001-07-01), Freitag et al.
patent: 6326155 (2001-12-01), Maclennan et al.
patent: 2002/0019517 (2002-02-01), Koide
patent: 2002/0137891 (2002-09-01), Hill et al.
patent: 0221175 (1991-02-01), None
patent: WO 00/34784 (2000-06-01), None
patent: WO 01/02440 (2001-01-01), None
Irwin et al., Affinity precipitation: a novel approach to protein purification, Essays Biochem. 1995;29:137-56.
Galaev, I. Yu., “New Methods of Protein Purification. Affinity Ultrafiltration,”Biochem.(Moscow) 64(8):849-856, 1999 (translated fromBiokhimiya(64(8):1013-1021).
Galaev I. and Mattiasson B., “‘Smart’ polymers and what they could do in biotechnology and medicine,”TIBTECH 17:335-340, 1999.
Hoffman et al., “Really smart bioconjugates of smart polymers and receptor proteins,” Founder's Award, Sixth World Biomaterials Congress 2000, Kamuela, HI, May 15-20,J. Biomed Mater Res. 52:577-586, 2000.
Irwin J. and Tipton K., “Affinity Precipitation Methods,”Methods in Molecular Biology, Ed: S. Doonan, Totowa, NJ: Humana Press Inc., vol. 59:217-238.
Larsson, P. and Mosbach, K., “Affinity Precipitation of Enzymes,”FEBS Letters:98(2): 333-338, 1979.
Hilbrig and Freitag, “Protein purification by affinity precipitation,”J Chromatogr B 790:79-90, 2003.
Labrou and Clonis, “The affinity technology in downstream processing,”J Biotechnol 36:95-119, 1994.
Roque et al., “Antibodies and genetically engineered related molecules: production and purification,”Biotechnol Prog 20:639-654, 2004.

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