Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-05
1997-12-23
Furman, Keith C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435233, 4352541, 4352523, 435325, 4353201, 435410, 536 232, 536 2374, C12N 1552, C12N 1531
Patent
active
057006598
ABSTRACT:
A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polyeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polylpeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.
REFERENCES:
Kaska et al., "Purification and characterization of protein disulphide-isomerase from the unicellular green alga Chlamydomonas reinhardii," BIOCHEM J., vol. 268, pp. 63-68, 1990.
Lee "Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase," SCIENCE vol. 239, pp. 1288-1291, Mar. 11, 1988.
Asami Osamu
Hirai Masana
Hoshino Fumihiko
Idekoba Chie
Imaeda Takao
Furman Keith C.
Kabushiki Kaisha Toyota Chuo Kenkusho
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