Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2007-05-01
2007-05-01
Rao, Manjunath (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S004000, C435S006120, C435S183000, C435S192000, C435S252300, C435S320100, C536S023200, C536S023400, C536S023500, C536S023700, C530S350000
Reexamination Certificate
active
10466085
ABSTRACT:
The object of the present invention is to provide a polypeptide which can be used to produce a saccharide having the structure of cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→}, a DNA encoding the polypeptide, and uses thereof. The present invention solves the above object by establishing a polypeptide which has an enzymatic activity of forming a saccharide with a glucose polymerization degree of 3 or higher and bearing both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end from a saccharide with a glucose polymerization degree of 2 or higher and bearing the α-1,4 glucosidic linkage as a linkage at the non-reducing end by α-glucosyl transferring reaction without substantially increasing the reducing power, a DNA encoding the polypeptide, a replicable recombinant DNA comprising the DNA encoding the polypeptide and an autonomously replicable vector, a transformant constructed by introducing the recombinant DNA into an appropriate host, and uses thereof.
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Fukuda Shigeharu
Kubota Michio
Maruta Kazuhiko
Yamamoto Takuo
Browdy and Neimark PLLC
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Rao Manjunath
LandOfFree
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