Polypeptide having α-isomaltosyl-transferase activity

Details Polypeptide having α-isomaltosyl-transferase activity Polypeptide having α-isomaltosyl-transferase activity

2006-08-29

2006-08-29

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S325000, C435S252310, C435S069100, C435S252330, C435S041000, C435S252200, C435S320100, C435S254200, C536S023200

Reexamination Certificate

active

07098013

ABSTRACT:
The object of the present invention is to provide a polypeptide which can be used to produce a saccharide having a structure of cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→}, a DNA encoding the polypeptide, and uses thereof. The present invention solves the above object by establishing a polypeptide which has an enzymatic activity to produce a saccharide having a structure of cyclo{→6}-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→} from a saccharide with a glucose polymerization degree of 3 or higher and bearing both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end by catalyzing the α-isomaltosyl-transfer, and having an amino acid sequence of either SEQ ID NO:1 or SEQ ID NO:2, or that which is a member selected from the group consisting of amino acid sequences having deletion, replacement, or addition of one or more amino acid residues therein or thereto, a DNA encoding the polypeptide, and uses thereof.

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