Polypeptide having Δ5 desaturating activity,...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S281000, C435S410000, C435S320100, C536S023200

Reexamination Certificate

active

07928294

ABSTRACT:
The present invention relates to a polypeptide having a Δ5 fatty acid desaturation activity and a polynucleotide encoding the polypeptide as well as use thereof. For example, the present invention relates to a polypeptide, comprising (a) the amino acid sequence represented by SEQ ID NO: 2; or (b) the amino acid sequence in which one or more amino acids are substituted, deleted, inserted or added in the amino acid sequence represented by SEQ ID NO: 2, and having the Δ5 fatty acid desaturation activity; an antibody capable of binding to this polypeptide; a polynucleotide encoding the polypeptide; a vector comprising this polynucleotide; a cell or transformant introduced with the polynucleotide; a method of producing a fatty acid using the cell, and so on.

REFERENCES:
patent: 6479070 (2002-11-01), Cain et al.
patent: 2001-158737 (2001-06-01), None
patent: WO 99/33958 (1999-07-01), None
patent: WO 00/20603 (2000-04-01), None
patent: WO 0138484 (2001-05-01), None
patent: WO 02/026946 (2002-04-01), None
Sequence alignment of SEQ ID No: 2 with Sequence Accession AAH50959, Lerchl et al, Aug. 28, 2001.
Sequence alignment of SEQ ID No: 1 with Sequence Accession AV644153, Asamizu et al, Dec. 15, 2000.
Hong et al., “Isolation and Characterization of a Δ5 FA Desaturase fromPythium irregulareby Heterologous Expression inSaccharomyces cerevisiaeand Oilseed Crops,” Lipids, vol. 37, No. 9, (2002), pp. 863-868.
Kajikawa et al., “Isolation and characterization of Δ6-desaturase, an ELO-like enzyme and Δ5-desaturase from the liverwortMarchantia polymorphaand production of arachidonic and eicosapentaenoic acids in the methylotrophic yeastPichia pastoris,” Plant Molecular Biology, 54, pp. 335-352, 2004.
International Search Report mailed on Oct. 18, 2005 in International PCT Application No. PCT/JP2005/013200 filed Jul. 11, 2005.
Masataka Kajikawa et al., “Ryokuso Chlamydomonas no Pinolenic Acid Namagosei o Ninau ω13 Fuhowaka Koso Idenshi no Tanri to Kino Kaiseki”, Annual Meeting of the Molecular Biology Society of Japan Program Koen Yoshishu, Dec. 2004, vol. 27, p. 850 3PA-163.
Christian Giroud et al., “Lipids ofChlamydomonas reinhardtii. Incorporation of [14C]Acetate, [14C]Palmitate and [14C]Oleate into Different Lipids and Evidence for Lipid-Linked Desaturation of Fatty Acids.”Plant Cell Physiology. 1989, vol. 30, No. 1, pp. 121-128.
Christian Giroud et al. Lipids ofChlamydomonas reinhardtii. Analysis of Molecular Species and Intracellular Site(s) of Biosynthesis.Plant Cell Physiology. 1988, vol. 29, No. 4, pp. 587-595.
Asamizu et al., “Generation of Expressed Sequences Tags from Low-CO2and High-CO2Adapted Cells ofChlamydomonos reinhardtii,” DNA Research, vol. 7, 2000, pp. 305-307, Kazusa DNA Research, Tokyo, Japan.
Grenier et al., Modification of the membrane fatty acid composition ofChlamydomonas reinhardtiicultured in the presence of liposomes, Plant Physiol. vol. 29, No. 5, 1991, pp. 429-440, Japanese Society of Plant Physiologists, Kyoto, Japan.
Domergue et al., “Cloning and functional characterization ofPhaeodactylum triconutumfront-end desaturases involved in eicosapentaenoic acid biosynthesis,” Eur. J. Biochem., vol. 269, 2002, Feb. 2002, Blackwell Science, Berlin, Germany.
European Search Report mailed on Apr. 28, 2008, in European PCT Application No. 05766483.1 dated Apr. 15, 2008.
Wolff et al., “Fokienia hodginsiiseed oil, another source of all-cis5,9,12,15-18:4 (coniferonic) acid,” Journal of American Oil Chemists' Society, vol. 76, No. 4, pp. 535-536, Apr. 1999.

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