Polypeptide having thrombin inhibiting activity

Details Polypeptide having thrombin inhibiting activity Polypeptide having thrombin inhibiting activity

1990-06-04

1992-11-24

Ellis, Joan

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530300, 435 6811, 4351723, 43525231, A61K 3514, A61K 3702, C12P 2100, C12N 1500

Patent

active

051663180

ABSTRACT:
Modified HV1-type hirudinin which valine at the N-terminal end of the HV1-type hirudin and aspartic acid at the 5th residue of the N-terminal end were replaced with alanine and glutamic acid, respectively. A secretion plasmid into which a DNA sequence coding for a precursor with an addition of a secretion signal of neutral protease of Bacillus amyloliquefaciens at the N-terminal end of this modified HV1-type hirudin is integrated is introduced into bacteria of the genus Bacillus and the precursor is expressed intracellularly. The modified HV1-type hirudin can be efficiently secreted extracellularly while maintaining its high thrombin inhibiting activity.

REFERENCES:
Wallace et al. Biochemistry vol. 28 pp. 10079-10084 (1989).
Harvey et al, Proc. Natl. Acad. Sci. USA vol. 83 pp. 1084-1088 (1986).
Dodt et al. FEBS. vol 202 pp. 373-377 (1986).
Honjo et al J. Biotechnol. vol. 4, pp. 63-71.
Nakayama et al, Journal of Biotechnology, vol. 8, No. 2, pp. 123-134, 1988.

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