Polypeptide from Humicola insolens possessing protein disulfide

Details Polypeptide from Humicola insolens possessing protein disulfide Polypeptide from Humicola insolens possessing protein disulfide

1993-05-27

1996-03-05

Jones, W. Gary

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

530324, 530350, 435183, C12N 990, C12N 900, A61K 3800, C07K 100

Patent

active

054967198

ABSTRACT:
A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polypeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polypeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4904602 (1990-02-01), Pigiet et al.
Gunther et al. J. of Biol. Chem. 268(11):7728 (1993).
Kaska et al. Biochem. J. 268:63 (1990).
Goldberger et al. J. of Biol. Chem. 238(2):628 (1963).
Edman et al. Nature 317:267 (1985).
Mizunga et al. J. Biochem. 108:846 (1990).
Tachikawa et al. J. Biochem 110:306 (1991).
LaMantia et al. P.N.A.S. 88:4453 (1991).
Scherens et al. Yeast 7:185 (1991).
Parkkonen et al. Biochem. J. 256:1005 (1988).
Yamauchi et al. Biochim & Biophy. Res. Comm. 146(3):1485 (1987).
Pihlajniemi et al. EMBO J. 6(3):643 (1987).
Gong et al. Nucleic Acid Res. 16(3)1203 (1988).

Affiliated with

Also associated with

Rating

Polypeptide from Humicola insolens possessing protein disulfide does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polypeptide from Humicola insolens possessing protein disulfide , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polypeptide from Humicola insolens possessing protein disulfide will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1412419