Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1992-01-31
1995-09-26
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530324, 530395, C07K 1300, C07K 710, A61K 3702
Patent
active
054534895
ABSTRACT:
In accordance with the present invention, there is provided a novel synthetic polypeptide derived from the first type III repeat of fibronectin. The synthetic polypeptide of the invention encompasses a fibronectin-fibronectin binding site, and is capable of inhibiting fibronectin matrix assembly. In contrast to previously identified fibronectin fragments that block fibronectin matrix assembly by blocking an initial event in matrix assembly (i.e., fibronectin binding to cells), the invention polypeptide appears to inhibit an intermediate step in matrix assembly, i.e., fibronectin self-association prior to the disulfide cross-linking that stabilizes the fibronectin matrix.
REFERENCES:
patent: 4578079 (1986-03-01), Ruoslahti et al.
patent: 4589881 (1986-05-01), Pierschbacher et al.
Hayashi, M., et al. (1981) J. Biol. Chem. 256: 11292-11300.
Calaycay, J., et al. (1985) J. Biol. Chem. 260: 12136-12141.
Akiyama et.al., Analysis of Fibronectin Receptor Function with Monoclonal Antibodies: Roles in Cell Adhesion, Migration, Matrix Assembly, and Cytoskeletal Organization. J.Cell Biol., 109: 863-875 (1991).
Ali and Hynes, Role of Disulfide Bonds in the Attachment and Function of Large, External, Transformation-Sensitive Glycoprotein at the Cell Surface. Biochim. Biophys. Acta. 510:140-150 (1978).
Chernousov et.al., Role of the I-9 and III-1 Modules of Fibronectin in Formation of an Extracellular Fibronectin Matrix. J. Biol. Chem. 266: 10851-10858 (1991).
Ehrismann et.al., Mode of Action of Fibronectin in Promoting Chicken Myoblast Attachment, Mr=60,000 gelatin-binding fragment binds native fibronectin. J. Biol. Chem. 256:4056-4062 (1981).
Ehrismann et.al., Arrangement of Attachment-Promoting, Self-Association, and Heparin-Binding Sites in Horse Serum Fibronectin. J. Biol. Chem. 257: 7381-7387 (1982).
Fogerty et.al., Inhibition of Binding of Fibronectin to Matrix Assembly Sites by Anti-integrin (alpha 5 beta 1) Antibodies. J. Cell Biol. 111: 699-708 (1990).
Giancotti and Ruoslahti, Elevated Levels of the Alpha 5 Beta 1 Fibronectin Receptor Suppress the Transformed Phenotype of Chinese Hamster Ovary Cells. Cell 60: 849-859 (1990).
Hynes and Destree, Extensive Disulfide Bonding at the Mammalian Cell Surface. Proc. Natl. Acad. Sci. U.S.A. 74:2855-2859 (1977).
Keski-Oja et.al., Dimeric Character of Fibronectin, a Major Cell Surface-Associated Glycoprotein. Biochem. Biophys. Res. Commun. 74: 699-706 (1977).
McDonald, J. A., Extracellular Matrix Assembly. Annu. Rev. Cell Biol. 4: 183-207 (1988).
McDonald et.al., Fibronectin Cell-Adhesive Domain and an Amino-Terminal Matrix Assembly Domain Participate in it Assembly into Fibroblast Pericellular Matrix. J. Biol. Chem. 262: 2957-2967 (1987).
McKeown-Longo et.al., Interaction of the 70,000-mol-wt Amino-terminal Fragment of Fibronectin with the Matrix-assembly Receptor of Fibroblasts. J. Cell Biol. 100: 364-374 (1985).
Mosher et.al., Assembly of Fibronectin into Extracellular Matrix. Ann. N.Y. Acad. Sci. 614: 167-180 (1991).
Nagai et.al., Monoclonal Antibody Characterization of Two Distant Sites Required for Function of the Central Cell-Binding Domain of Fibronectin in Cell Adhesion, Cell Migration, and Matrix Assembly. J. Cell Biol. 114: 1295-1305 (1991).
Oh et.al., Deposition of Plasma Fibronectin in Tissues. Prod. Natl. Acad. Sci. U.S.A. 78: 3218-3221 (1981).
Peters and Mosher, Localization of Cell Surface Sites Involved in Fibronectin Fibrillogenesis. J. Cell Biol. 104: 121-130 (1987).
Peters et.al., Co-assembly of Palsma and Cellular Fibronectins into Fibrils in Human Fibroblast Cultures. J. Cell Biol. 111: 249-256 (1990).
Pierschbacher et.al., Cell Attachment Activity of Fibronectin can be Duplicated by Small Synthetic Fragments of the Molecule. Nature 309: 30-33 (1984).
Pierschbacher et.al., Synthetic Peptide with Cell Attachment Activity of Fibronectin. Proc. Natl. Acad. Sci. U.S.A. 80: 1224-1227 (1983).
Pierschbacher et.al., Variants of the Cell Recognition Site of Fibronectin That Retain Attachment-promoting Activity. Proc. Natl. Acad. Sci. U.S.A. 81: 5985-5988 (1984).
Pytela et.al., Identification and Isolation of a 140 kd Cell Surface Glycoprotein with Properties Expected of a Fibronectin Receptor. Cell 40: 191-198 (1985).
Quade and McDonald, Fibronectin's Amino-terminal Matrix Assembly Site is Located Within the 29-kDa Amino-terminal Domain Containing Five Type I Repeats. J. Biol. Chem. 263: 19602-19609 (1988).
Ruoslahti, E., Fibronectin and its Receptors. Annu. Rev. Biochem. 57: 375-413 (1988).
Ruoslahti, E., Integrins. J. Clin. Invest. 87:1-5 (1991).
Schwarzbauer, J. E., Identification of the Fibronectin Sequence Required for Assembly of a Fibrillar Matrix. J. Cell Biol. 113: 1463-1473 (1991).
Schwarzbauer, et.al., Efficient and Stable Expression of Recombinant Fibronectin Polypeptides. Proc. Natl. Acad. Sci. U.S.A. 84: 754-758 (1987).
Woods et.al., Fibronectin Fibril Formation Involves Cell Interactions With Tow Fibronectin Domains. Exp. Cell Res. 177: 272-283 (1988).
Morla Alex
Ruoslahti Erkki I.
Draper Garnette D.
Fitzgerald David L.
La Jolla Cancer Research Foundation
LandOfFree
Polypeptide fragments of fibronectin which can modulate extracel does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polypeptide fragments of fibronectin which can modulate extracel, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polypeptide fragments of fibronectin which can modulate extracel will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1552491