Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1999-02-17
2000-11-28
Carlson, Karen Cochrane
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
435 691, 43525231, 4353201, 435410, 536 2371, 536 241, 530350, 574 2, 574 12, A01H 104, C12P 2106, C07H 1700, C07K 1400
Patent
active
061538140
ABSTRACT:
Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
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Baum James A.
Gilmer Amy Jelen
Mettus Anne-Marie Light
Ball, Esq. Timothy K.
Carlson Karen Cochrane
Monsanto Company
Simon Howrey
LandOfFree
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