Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Reexamination Certificate
2006-05-30
2006-05-30
Le, Long V. (Department: 1641)
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
C436S056000, C436S057000, C436S019000, C436S120000, C436S173000, C436S161000, C435S007100, C435S007920, C435S024000
Reexamination Certificate
active
07052916
ABSTRACT:
Methods for incorporating a stable isotope into a protein or peptide fragment are disclosed. The methods include providing isolated isotope-labeled protein or peptide fragments and analyzing the isolated labeld fragments. In another aspect of the invention, methods for quantitatively comparing peptides in two protein or peptide fragment mixtures are provided. In these methods, protein levels are measured using stable-isotope coded protein or peptide fragments.
REFERENCES:
patent: 5986061 (1999-11-01), Pestka
patent: 6348569 (2002-02-01), Walker et al.
patent: 6391650 (2002-05-01), Anderson et al.
patent: 6670194 (2003-12-01), Aebersold et al.
patent: 2003/0108959 (2003-06-01), Johnson et al.
Kanstrup et al.. Arch Biochem & Biophysics 1993 vol. 304, pp. 332-337.
Adamczyk, M. et al., “Selective analysis of phosphopeptides within a protein mixture by chemical modification, reversible biotinylation and mass spectrometry,”Rapid Commun. Mass Spectrom., 15:1481-1488, 2001.
Byford, M., “Rapid and selective modification of phosphoserine residues catalyzed by Ba2+ions for their detection during peptide microsequencing,”Biochem. J., 280:261-265, 1991.
Goshe, M. et al., “Phosphoprotein isotope-coded affinity tag approach for isolating and quantitating phosphopeptides in proteome-wide analyses,”Anal. Chem., 73:2578-2586, 2001.
Gygi, S. et al., “Quantitative analysis of complex protein mixtures using isotope-coded affinity tags,”Nature Biotech., 17:994-999, 1999.
Jaffe, H. et al., “Characterization of serine and threonine phosphorylation sites in β-elimination/ethanethiol addition-modified proteins by electrospray tandem mass spectrometry and database searching,”Biochem., 37:16211-16224, 1998.
Johnson, R. et al., “Isotope Coded Labile Affinity Proteomics,” Poster presented at the 49thAmerican Society Mass Spectrometry, on Mass Spectrometry and Allied Topics, in Chicago, IL, May 27-31, 2001.
Oda, Y. et al., “Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome,”Nature Biotech., 19:379-382, 2001.
Qian, W. et al., “Phosphoprotein isotope-coded solid-phase tag approach for enrichment and quantitative analysis of phosphopeptides from complex mixtures,”Anal. Chem., 75:5441-5450, 2003.
Qiu, Y. et al., “Acid-labile isotope-coded extractants: A class of reagents for quantitative mass spectrometric analysis of complex protein mixtures,”Anal. Chem., 74:4969-4979, 2002.
Shen, M. et al., “Isolation and isotope labeling of cysteine- and methionine-containing tryptic peptides,”Molecular&Cellular Proteomics2:315-324, 2003.
Spahr, C. et al., “Simplification of complex peptide mixtures for proteomic analysis: Reversible biotinylation of cysteinyl peptides,”Electrophoresis, 21:1635-1650, 2000.
Weckwerth, W. et al., “Comparative quantification and identification of phosphoproteins using stable isotope labeling and liquid chromatography/mass spectrometry,”Rapid Commun. Mass Spectrom., 14:1677-1681, 2000.
Cheu Jacob
Christensen O'Connor Johnson & Kindness PLLC
Immunex Corporation
Le Long V.
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