Polynucleotides for the detection and quantification of...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Details

C530S300000, C435S005000, C435S006120, C435S004000, C435S029000, C435S034000, C435S039000, C536S024330

Reexamination Certificate

active

07015317

ABSTRACT:
The present invention provides polynucleotides that can specifically hybridise to Hepatitis B virus (HBV) nucleic acids from all genotypes. These polynucleotides can be used in genotype-independent detection and quantitation of HBV nucleic acids. For example, the polynucleotides can be used as primers and/or probes in amplification-based assays for either end-point detection or real-time monitoring of HBV nucleic acids in a test sample. The polynucleotides can additionally be provided as part of a kit for the detection and quantitation of HBV nucleic acids.

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Sequence alignment of SEQ ID No. 1 with residues 15-40 of SEQ ID No.: 22 of Spies.
Sequence alignment of SEQ ID No. 3 with Residues 35-9 of SEQ ID No.: 23 of Spies.
Sequence alignment of SEQ ID No. 1 with Residues 246-271 of SEQ ID No.: 11 and residues 1652-1677 of SEQ ID No.: 15 of Rutter et al.
Sequence alignment of SEQ ID No. 3 with Residues 438-412 of SEQ ID No.: 11 and residues 1844-1818 of SEQ ID No.: 15 of Rutter et al.
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Marras et al. “Multiplex detection of single-nucleotide variations using molecular beacons”, Genetic Analysis: Biomolecular Engineering, USA, pp. 151-156 (1999).

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