Polynucleotides encoding molecular complexes which modify immune

Chemistry: molecular biology and microbiology – Vector – per se

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536 231, 536 234, 536 235, 536 2353, 536 242, 4353201, 435325, 4352523, 4352542, C12N 1563, C12N 1509, C12N 510, C12N 1566, C07H 2100

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061401137

ABSTRACT:
Polynucleotides encode soluble, multivalent molecular complexes which modify immune responses, and host cells comprise such polynucleotides. The molecular complexes comprise extracellular domains of transmembrane heterodimeric proteins, particularly T cell receptor and major histocompatibility complex proteins, which are covalently linked to the heavy and light chains of immunoglobulin molecules to provide soluble multivalent molecular complexes with high affinity for their cognate ligands. The molecular complexes can be used, inter alia, to detect and regulate antigen-specific T cells and as therapeutic agents for treating disorders involving immune system regulation, such as allergies, autoimmune diseases, tumors, infections, and transplant rejection.

REFERENCES:
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T. Johansen et al. "Potent inhibition of alloreactive T cells by nanomolar concentrations of a divalent soluble class I MHC molecule" The Journal of Immunology, vol. 150, No. 8, part 2, Apr. 15, 1993, p. 83A.
C. Gregoire et al. "Engineered secreted T-cell receptor alpha-beta heterodimers" Proceedings of the National Academy of Science of the USA vol. 88, No. 18, Sep. 15, 1991, pp. 8077-8081.
D. Eilat et al. "Secretion of a soluble, chimeric gamma-delta T-cell receptor-immunoglobulin heterodimer" Proceedings of the National Academy of Sciences of the USA, vol. 89, No. 15, Aug. 1, 1992, pp. 6871-6875.
S. Weber et al. "Specific low-affinity recognition of major histocompatibility complex plus peptide by soluble T-cell receptor" Nature, vol. 356, No. 6372, Apr. 30, 1992, pp. 792-976.
H-C Chang et al. "A general method for facilitating heterodimeric pairing between two proteins: Application to expression of alpha and beta T-cell receptor extracellular segments" Proceedings of the National Academy of Sciences of the USA, vol. 91, Nov.1994, pp. 11408-11412.
S. O'Herrin et al. "Expression and analysis of soluble MHC- and TcR-immunoglobulin super dimers" The FASEB Journal, vol. 10, No. 6, Apr. 30, 1996 p. A1473.
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