Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2007-02-13
2007-02-13
Swope, Sheridan (Department: 1656)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C435S320100, C435S252300, C435S252330, C435S325000, C435S348000
Reexamination Certificate
active
09858332
ABSTRACT:
The present invention provides metal ion affinity peptides, fusion proteins comprising metal ion affinity peptides, and polynucleotides encoding the fusion proteins. A feature of the subject invention is that the metal ion affinity peptide has a formula selected from the group consisting of: formula 1: (His-X1-X2)n1-(His-X3-X4-X5)n2-(His-X6)n3, wherein each of X1and X2is independently an amino acid with an aliphatic or an amide side chain, each of X3, X4, X5is independently an amino acid with a basic side chain (except His) or an acidic side chain, each X6is an amino acid with an aliphatic or an amide side chain, n1 and n2 are each independently 1–3, and n3 is 1–5; formula 2: (His-Asn)n, where n=3 to 10; and formula 3: (His-X1-X2)n, wherein each of X1and X2is an amino acid having an acidic side chain, and n=3 to 10. The invention further provides recombinant vectors comprising subject polynucleotides, and host cells comprising the recombinant vectors. The invention further provides methods and kits for purifying a fusion protein comprising a metal ion affinity peptide.
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Jokhadze George G.
Tchaga Grigoriy S.
Bozicevic Field & Francis LLP
Clontech Laboratories, Inc.
Field Bret E.
Scherer David C.
Swope Sheridan
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