Polynucleotides encoding human sphingosine Lyase

Details Polynucleotides encoding human sphingosine Lyase Polynucleotides encoding human sphingosine Lyase

1999-01-27

2001-02-13

Duffy, Patricia A. (Department: 1647)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C536S023200, C435S320100, C435S232000, C435S252300, C435S252330, C435S070100, C435S071100, C435S476000, C435S478000

Reexamination Certificate

active

06187562

ABSTRACT:

This application claims the benefit of prior EP Patent Application Serial No. 98300625.5 filed Jan. 29, 1998, and UK Patent Application Serial No. 9824026.0, filed Nov. 3, 1998.
FIELD OF THE INVENTION
This invention relates to newly identified polypeptides and polynucleotides encoding such polypeptides, to their use in therapy and in identifying compounds which may be agonists, antagonists and/or inhibitors which are potentially useful in therapy, and to production of such polypeptides and polynucleotides.
BACKGROUND OF THE INVENTION
The drug discovery process is currently undergoing a fundamental revolution as it embraces ‘functional genomics’, that is, high throughput genome- or gene-based biology. This approachas a means to identify genes and gene products as therapeutic targets is rapidly superseding earlier approaches based on ‘positional cloning’. A phenotype, that is a biological function or genetic disease, would be identified and this would then be tracked back to the responsible gene, based on its genetic map position.
Functional genomics relies heavily on high-throughput DNA sequencing technologies and the various tools of bioinformatics to identify gene sequences of potential interest from the many molecular biology databases now available. There is a continuing need to identify and characterise further genes and their related polypeptides/proteins, as targets for drug discovery.
SUMMARY OF THE INVENTION
The present invention relates to SPHINGLY, in particular SPHINGLY polypeptides and SPHINGLY polynucleotides, recombinant materials and methods for their production. In another aspect, the invention relates to methods for using such polypeptides and polynucleotides, including the treatment of cancers, cardiovascular disorders, thrombosis, atherosclerosis, wound healing, stroke, CNS disorders, neuronal disorders and apoptosis, hereinafter referred to as “the Diseases”, amongst others. In a further aspect, the invention relates to methods for identifying agonists and antagonists/inhibitors using the materials provided by the invention, and treating conditions associated with SPHINGLY imbalance with the identified compounds In a still further aspect, the invention relates to diagnostic assays for detecting diseases associated with inappropriate SPHINGLY activity or levels.


REFERENCES:
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Jenkins et al, PCR Methods and Applications, S77-81, 1994.
Zhou et al. “Identification of the First Mammalian Sphingosine Phosphate Lyase Gene and Its Functional Expression in Yeast”, Biochem. Res. Comm., vol. 242, pp. 502-507 (1998).
Saba et al. “The BSTI Gene ofSaccharomyces cerevisiaeIs the Sphingosine-1-phosphate Lyase”, J. Biol. Chem., vol. 272, pp. 26807-26090 (1997).
GenBank Accession #AF036894. Created Feb. 24, 1998 Zhov et al; Marra et al; Zhov et al.
GenBank Accession #U51031. Created Mar. 23, 1996 Fulton L; Johnston et al; Waterston R; Jia et al.

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