Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2000-05-12
2002-11-05
Duffy, Patricia A. (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069700, C435S070100, C435S071100, C435S320100, C435S325000, C435S252300, C435S254110, C435S257300, C530S350000, C536S023400, C536S023700, C536S024320, C536S024330
Reexamination Certificate
active
06475754
ABSTRACT:
BACKGROUND OF THE INVENTION
Bordetella bronchiseptica
is an etiologic agent of infectious tracheobronchitis (Kennel Cough) in dogs, atrophic rhinitis in pigs, and respiratory infections in rabbits and guinea pigs. Bordetella infections depress the respiratory clearance mechanisms (mucociliary elevator), facilitating invasion by other organisms. Colonization of the upper respiratory tract by the bacteria occurs via firm attachment to the ciliated respiratory epithelium, followed by rapid proliferation and an inflammatory response. Binding to host tissue is the primary step in the establishment of infection, and
B. bronchispetica
produce an array of moleculesthat mediate attachment, including filamentous hemagglutinin, tracheal cytotoxin, pertactin, and fimbriae. Several genes coding for fimbrial subunits have been previously reported, including fim 2, fim 3, fim A, and fim X. Most of these fimbrae have counterparts in
B. pertussis.
Two fimbrial phenotypes of
B. bronchiseptica
have been defined serologically as fim 2 and fim 3. They are reactive with factor specific antiserums historically used for serotyping
B.pertussis
isolates. Fim A and fim X (Boschwitz, J. S., H. G. J. van der Heide, F. R. Mooi, and D. A. Relman [1997]
J. Bacteriol.
179:7882-7885), two other major fimbrial proteins, are also expressed in
B.bronchiseptica;
however, their antigenic characteristics are not known. Existence of other bordetella fimbriae has been hypothesized (Mooi, F. R., W. H. Jansen, H. Brunings, H. Gielen, H. G. J van der Heide, H. C. Walvoort, and Guinee, P. A. M. [1992]
Micro. Pathog.
12:127-135).
The expression of bordetella fimbrial genes is positively regulated through the bvg locus (Willems, R., A. Paul, H. G. J. van der Heide, A. R. ter Avest, and F. R. Mooi [1990]
EMBO J.
9:2803-2809) and their promoter regions have in common a stretch of cytosines referred to as a C-stretch (Willems et al. [1990] supra; Riboli, B., P. Pedroni, A. Cuzzoni, and F. de Ferra [1991]
Micro. Pathog.
10:393-403), and two motifs 5′ to the C-stretch comprising an ABR or fim box (Riboli et al., [1991] supra). Characterization of fimbrial genes and their products is important to the understanding of fimbrial gene regulation, for serological diagnosis, for identification of potential subunit vaccine candidates, and to understand mechanisms of cellular adhesion.
BRIEF SUMMARY OF THE INVENTION
The subject invention concerns novel polynucleotides of Bordetella species, and the polypeptides encoded thereby. The subject invention further provides vaccines useful for providing protection against Bordetella infections.
In a specific embodiment, the subject invention provides a unique
Bordetella bronchiseptica
gene. This gene was isolated from a cosmid constructed with
B. bronchiseptica
chromosomal DNA. This cosmid, when introduced into attachment deficient
B. bronchiseptica
, increases the binding of the bacteria to epithelial cells. The fimbrial gene, referred to as fim N, has been subcloned and sequenced. The predicted protein from this gene has about 59% and about 52% homology with
B. bronchiseptica
fim 2 and fim 3, respectively. Fim N encodes a protein predicted to be similar in size and structure to those encoded by fim 2 and fim 3. Serologically, the fim N protein appears to have cross-reactivity with serotype 2 antibodies. The fim N protein is present in
B. bronchiseptica
isolates associated with disease in several species of veterinary importance.
Various abbreviations are found within this specification. Their terms and meanings are as follows: aa, amino acid(s); Ab, antibody(ies); Ap, ampicillin; ELISA, enzyme-linked immunosorbent assay; EtdBr, ethidium bromide; IPTG, isopropyl &bgr;-D-thiogalactopyranoside; kb, kilobase; LB, Luria-Bertani (medium); mAB, monoclonal Ab; Ni—-NTA, nickel-nitrilotriacetic acid; nt, nucleotide(s); O.D., optical density; oligo, oligonucleotide; ORF, open reading frame; p, plasmid; PCR, polymerase chain reaction; PBS, phosphate buffered saline; PBSTW, PBS containing polyoxyethylenesorbitan monolaurate; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; Tc, tetracycline.
REFERENCES:
patent: 5939064 (1999-08-01), Savelkoul et al.
Sambrouk et al, in “Molecular Cloning A Labortory Manual”, pp. 17.2-17.43 Cold Spring Harbor Press Inc., 1989.*
Gerhold et al, BioEssays. 18(12):973-981, 1996.*
Wells et al, J. of Leukayte Biology 61(5):545-550, 1997.*
Russell et al, Journal of Molecular Biology 244:332-350, 1994.*
Rudinger, in “Pepticle Hormones” ed. Parsons, J.A., University Park Press, pp. 1-6, Jun. 1976.*
Burgess et al, The Journal of Cell Biology, 111:2129-2138, 1990.*
Lazar et al, Molecular and Cellular Biology, 8(3):1247-1252, 1988.*
Jobling et al, Mol. Microbiol, 5(7):1755-67, 1991.*
Boehnager Mannheim Biochemsitry, 1991 catalog. p. 557, 1991.*
Stratagene, 1991 Product Catalog, p. 66, 1991.*
Gibcobiol., Catalogue & Reference Guide 1992 p. 292.*
Pronega (1993/1994 Catalog) pp. 90-91.*
New England Biolabs Catalog, 1986/1987 pp. 60-62.*
Kania, S.A., et al. [2000] “Characterization of fimN, a newBordetella bronchisepticamajor fimbrial subunit gene,”Gene256:149-55.
Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, David J. Lipman (1990) “Basic Local Alignment Search Tool”J. Mol. Biol.215(403-410.
Boschwitz, Jeffrey S., Han G. J. van Der Heide, Frits R. Mooi, David A. Relman (1997) “Bordetella bronchisepticaExpresses the Fimbrial Structural Subunit Gene fimA”Journal of Bacteriology179(24):78827885.
Burns, Jr., Eugene H., John M. Norman, Monty D. Hatcher, David A. Bemis (1993) “Fimbriae and Determination of Host Species Specificity ofBordetella bronchiseptica”Journal of Clinical Microbiology31(7):1838-1844.
Cox, Graham J. M., Tim J. Zamb, Lorne A. Babiuk (1993) “Bovine Herpesvirus 1: Immune Responses in Mice and Cattle Injected with Plasmid DNA”Journal of Virology67(9):5664-5667.
Meade, Harry M., Sharon R. Long, Gary B. Ruvkun, Susan E. Brown, Fred M. Ausubel (1982) “Physical and Genetic characterization of Symbiotic and Auxotrophic Mutants ofRhizobium melilotiInduced by Transposon Tn5 Mutagenesis”Journal of Bacteriology149:114-122.
Mooi, Frits R., Wim H. Jansen, Henk Brunings, Henk Gielen, Han G. J. van der Heide, Henk C. Walvoort and Piet A.M. Guinee (1992) “Construction and analysis ofBordetella pertussismutants defective in the production of fimbriae”Microbiol Pathogenesis12:127-135.
Riboli, B. P. Pedroni, A. Cuzzoni, G. Grandi and F. de Ferra (1991) “Expression ofBordetella pertussisfimbrial (fim) genes inBordetella bronchiseptica: fimX is expressed at a low level and vir-regulated”Microbial Pathogenesis10:393-403.
Sedegah, Martha, Richard Hedstrom, Peter Hobart, and Stephen L. Hoffman (1994) “Protection against malaria by immunization with plasmid DNA encoding circumsporozoite protein”Proc Natl. Acad. Sci. USA91:9866-9870.
Weiss, Alison A., Stanley Falkow (1982) “Plasmid Transfer toBordetella Pertussis: Conjugation and Transformation”Journal of Bacteriology152(1):549-552.
Willems, Rob, Annet Paul, Han G.J. vn der Heide, Anja R.ter Avest and Frits R. Mooi (1990) “Fimbrial phase variation inBordetella pertussis: a novel mechanism for transcriptional regulation”The EMBO Journal9(9):2803-2809.
F. Schödel, M.-T. Aguado and P.-H. Lambert (1994) Special Conference “Introduction: Nucleic Acid Vaccines, WHO, Geneva, May 17-18, 1994”Vaccine12:1491.
Bemis David A.
Kania Stephen A.
Moore Robert N.
Duffy Patricia A.
Saliwanchik Lloyd & Saliwanchik
University of Tennessee Research Corporation
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