Polynucleotides encoding anti-sulfotyrosine antibodies

Details Polynucleotides encoding anti-sulfotyrosine antibodies Polynucleotides encoding anti-sulfotyrosine antibodies

2011-01-11

2011-01-11

Wen, Sharon (Department: 1644)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S326000

Reexamination Certificate

active

07868152

ABSTRACT:
The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

REFERENCES:
patent: 5716836 (1998-02-01), Suiko
Hoffhines et al. (J. Biol. Chem. 2006, 281:37877-37887).

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