Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-08-18
1997-01-07
Zitomer, Stephanie W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 232, C07H 2102, C07H 2104
Patent
active
055918395
ABSTRACT:
Polynucleotide sequences encoding enzymes that possess .alpha.2-3 neuraminidase activity are provided. Of particular interest are polynucleotide sequences encoding an enzyme having .alpha.2-3 neuraminidase activity and naturally produced by the bacteria Streptococcus pneumoniae. Recombinant DNA expression of enzymes possessing .alpha.2-3 specific neuraminidase activity is also described, including methods, recombinant host cells and a genetic construction. The invention also provides a purified enzyme having .alpha.2-3 neuraminidase activity from Streptococcus pneumoniae, wherein the enzyme is isolated from S. pneumoniea cultures. Another aspect of this invention is to provide methods of isolating genes encoding enzymes having neuraminidase activity, particularly .alpha.2-3 neuraminidase activity. The gene isolation methods of the invention comprise the step of labeling a hybridization probe derived from the neuraminidase coding portion of plasmid pND-1. The hybridization probe may then be used to isolate homologous genes encoding enzymes having the desired enzymatic activity.
REFERENCES:
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Yeung et al, Applied. Envir. Microbiol 57: 3062-3069 (1991).
Davis et al, PNAS 80: 3976-3980 (1983).
Rademacher et al., Ann. Rev. Biochem., 57:789:838 (1988).
Advertisement entitled, "With Glyco FACE technology you can separate, quantify, or sequence your carbohydrate . . . In one day.," Nature, vol. 364, No. 6437, Reader Service No. 356 (Aug. 5, 1993).
Glyco, Inc., Product Specification for "NANase I . . . a recombinant glycosidase specific for .alpha.2-3 linked N-acetylneuraminic acid", pp. 1-2 (Jun. 20, 1994).
Klock John C.
Miller Harvey I.
Starr Christopher M.
Campbell Eggerton
Glyko, Inc.
Halluin, Esq. Albert P.
Zitomer Stephanie W.
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