Polynucleotides and their use for detecting resistance to...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S091200, C435S091100, C435S007340, C435S975000, C536S023100, C536S024300, C536S024320, C536S024330, C536S023700

Reexamination Certificate

active

06936422

ABSTRACT:
The present invention pertains to polynucleotides derived fromstaphylococcalgenes encoding resistance to streptogramin A or to streptogramin B and chemically related compounds. This invention also relates to the use of the polynucleotides as oligonucleotide primers or probes for detectingStaphylococcalstrains that are resistant to streptogramin A or to streptogramin B and related compounds in a biological sample. In another embodiment, the present invention is directed to the full length coding sequences of thestaphylococcalgenes encoding for resistance to streptogramin A or to streptogramin B fromStaphylococcusand to the polypeptide expressed by these full length coding sequences. Further, this invention relates to the use of the expressed polypeptides to produce specific monoclonal or polyclonal antibodies that serve as detection means in order to characterize anystaphylococcalstrain carrying genes encoding resistance to streptogramin A or to streptogramin B.

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