Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2007-12-04
2007-12-04
Navarro, Mark (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C530S300000, C424S185100, C424S190100, C424S234100
Reexamination Certificate
active
10696019
ABSTRACT:
We have successfully sequenced and cloned the gene expressing the Major Surface Protein 5 (MSP5) ofA. phagocytophilum. The recombinant MSP5 (rMSP5) protein has been tested using sera from humans and dogs infected withA. phacytophilum. The polypeptide has been found to be immunogenic and useful as a diagnostic test antigen. The polypeptide antigen of the subject invention can provide the basis of a diagnostic assay that would allow the rapid, in-house, laboratory diagnosis of infection withA. phagocytophilumusing a sample (e.g., serum, plasma, or whole blood) from an infected human or animal. Additionally, the subject invention provides methods of detecting the presence ofA. phagocytophilumin biological or environmental samples utilizing antibodies provided by the subject invention. Furthermore, the use of the single antigen in the diagnosis of this important disease offers many advantages including enhanced test specificity, ease of testing and consistency of results using synthetically produced test antigens instead of cultured, whole organisms.
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Alleman Arthur Rick
Barbet Anthony F.
Navarro Mark
Saliwanchik Lloyd & Saliwanchik
University of Florida Research Foundation Inc.
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