Polynucleotide reagent containing chiral subunits and methods of

Details Polynucleotide reagent containing chiral subunits and methods of Polynucleotide reagent containing chiral subunits and methods of

1993-02-09

1996-05-28

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

544 81, 544 82, 544118, 544123, 528398, 528399, 528403, 528405, 528406, C12Q 168, C07D41300, C08G 7902, C08G 5900

Patent

active

055210635

ABSTRACT:
The present invention describes an assay system wherein target polynucleotide molecules are captured on a support by base-specific binding to support-bound polymers, which are themselves substantially uncharged, and the target polynucleotides can be detected on the basis of their backbone charge. The assay system may also include polycationic reporter molecules which are designed to bind to the fully charged analyte backbone, but not the uncharged (or substantially uncharged) polymer backbone. In one embodiment, the reporter molecules are composed of a polycationic moiety or tail designed to bind electrostatically to a fully charged polynucleotide, under conditions where the reporter does not bind to the less charged or uncharged binding polymer carried on the diagnostic reagent.

REFERENCES:
patent: 4123610 (1978-10-01), Summerton et al.
patent: 4362531 (1982-12-01), de Steenwinkel et al.
patent: 4395486 (1983-07-01), Wilson et al.
patent: 4430496 (1984-02-01), Abbott
patent: 4507433 (1985-03-01), Miller et al.
patent: 5034506 (1991-07-01), Summerton et al.
patent: 5142047 (1992-08-01), Summerton et al.
patent: 5217866 (1993-06-01), Summerton et al.
patent: 5235033 (1993-08-01), Summerton et al.
Miller, P. S., et al., "Nonionic Nucleic Acid Analogues. Synthesis and Characterization of Dideoxyribonucleoside Methylphosphonates," Biochemistry 18(23):5134-5143 (1979).
Miller, P. S., et al., "Oligothymidylate Analogues Having Stereoregular, Alternating Methylphosphonate/Phosphodiester Backbones," The Journal of Biological Chemistry 255(20):9659-9665 (1980).
Miller, P. S., et al., "Biochemical and Biological Effects of Nonionic Nucleic Acid," Biochemistry 20:1874-1880 (1981).
Murakami, A., et al., "Characterization of Sequence-Specific Oligodeoxyribonucleoside Methylphosphonates and their Interaction with Rabbit Globin mRNA," Biochemistry 24:4041-4046 (1985).
Renz, M., and C. Kurz, "A colorimetric method for DNA hybridization," Nucleic Acids Research 12(8):3435-3444 (1984).
Stirchak, E. P., and J. E. Summerton, "Uncharged Stereoregular Nucleic Acid Analogues. 1. Synthesis of a Cytosine-Containing Oligomer with Carbamate Internucleoside Linkages," The Journal of Organic Chemistry 52:4202-4206 (1987).
Summerton, J. E., "Intracellular inactivation of specific nucleotide sequences: a general approach to the treatment of viral diseases and virally-mediated cancers," Abstract No. 91:151527g from Chemical Abstracts 91:73-74 (1979).

Affiliated with

Also associated with

Rating

Polynucleotide reagent containing chiral subunits and methods of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polynucleotide reagent containing chiral subunits and methods of, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polynucleotide reagent containing chiral subunits and methods of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-785880