Polynucleotide ligands as anti-viral agents

Details Polynucleotide ligands as anti-viral agents Polynucleotide ligands as anti-viral agents

2005-02-01

2005-02-01

Guzo, David (Department: 1636)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C536S023100

Reexamination Certificate

active

06849610

ABSTRACT:
Infectious human cytomegalovirus (HCMV) were isolated in vitro from a pool of randomized sequences after sixteen or 21 cycles of selection and amplification. The ligands characterized exhibited high HCMV-binding affinity in vitro and effectively inhibited viral infection in tissue culture. Several ligands blocked viral entry. Their antiviral activity was also specific as the ligands only reacted with strains of HCMV, but not with the related herpes simplex virus 1 and human cells. Moreover, the ligands recognize several different epitopes. Thus, RNA ligands can function to bind to a human virus and block viral infection. The screening method may utilize the novel features of binding to intact infectious virus, partitioning the bound polynucleotides from unbound by passing through a porous filter, and enhancing the release of bound polynucleotides by treatment with protease.

REFERENCES:
patent: 5523389 (1996-06-01), Ecker et al.
patent: 5856085 (1999-01-01), Wang et al.
Pan et al., Isolation of virus-neutralizing RNAs from a large pool of random sequences, 1995, Proc. Natl. Acad. Sci. USA, vol. 92, pp. 11509-11513.*
Wyllie, Susan (1999), “Single Channel Analysis of Recombinant Major Outer membrane Protein Porins from Chlamydia Psittaci and Chlamydia Pneumoniae.”FEBS Letters,vol. 445:192-196.
Allen, Judith (Jul. 15, 1991), “A Single Peptide from the Major Outer Membrane Protein of Chlamydia Trachomatis Elictis T Cell Help for the Production of Antibodies to Protective Determinants.”Journal of Immunology,vol. 147:674-679.
Wyllie, Susan (Nov. 1998), “The Major Outer Protein of Chlamydia Psittaci Functions as a Porin-like Ion Channel.”Infection and Immunity,pp. 5202-5207.
Ellington et al. (1990) “In vitro selection of RNA molecules that bind specific ligands.”Nature,vol. 346:818-22.
Gold et al. (1995) “Diversity of Oligonucleotide Functions.”Annu. Rev. Biochem.,vol. 64:763-97.
Tuerk et al. (1990) “Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T5 DNA Polymerase.”Science,vol. 249(4968):505-510.
Yang et al. (1998) “DNA ligands that bind tightly and selectively to cellobiose.”Proc. Natl. Acad. Sci. USA,vol. 95:5462-5467.

Affiliated with

Also associated with

Rating

Polynucleotide ligands as anti-viral agents does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polynucleotide ligands as anti-viral agents, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polynucleotide ligands as anti-viral agents will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3475977