Polynucleotide encoding osmotin modified for apoplast targeting

Details Polynucleotide encoding osmotin modified for apoplast targeting Polynucleotide encoding osmotin modified for apoplast targeting

1995-05-31

2000-07-11

Nelson, Amy

Chemistry: molecular biology and microbiology

Vector, per se

435440, 536 236, C12N 1529, C12N 1582

Patent

active

06087161&

ABSTRACT:
There is provided (a) a process to discharge into an extracellular space of a plant an intracellular osmotin protein that naturally has a vacuolar targeting sequence at its C-terminal end which naturally targets the protein into a plant vacuole; and (b) a recombinant polynucleotide for use in the process. The recombinant polynucleotide includes a promoter that is functional in plants; an open reading frame encoding an intracellular osmotin protein under control of the promoter, the open reading frame having been modified to target the intracellular osmotin protein to an apoplast by creating a translation stop codon in the open reading frame at the 3' end resulting in deletion of the C-terminal amino acids of the intracellular osmotin protein necessary for intracellular osmotin targeting; and a terminator operably linked to the modified open reading frame.

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