Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2011-07-12
2011-07-12
Steadman, David J (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S320100, C435S252300, C435S069100, C435S252100, C536S023200, C536S023700
Reexamination Certificate
active
07977073
ABSTRACT:
The present invention relates to an amide hydrolase which is with excellent thermostability and stereoselectively hydrolyzes an α-amino acid amide; a gene encoding the enzyme protein; a novel recombinant vector containing the gene; a transformant containing the recombinant vector; and a process for producing an L-α-amino acid using the transformant.
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Tae Kyou Cheong, et al., “Cloning of a Wide-Spectrum Amidase fromBacillus stearothermophilusRB388 inEscherichia coliand Marked Enhancement of Amidase Expression Using Directed Evolution”, Enzyme and Microbial Technology, vol. 26, No. 2-4, XP-002304495, Feb. 2000, pp. 152-158.
W. J. J. Van Den Tweel, et al., “Ochrobactrum anthropiNCIMB 40321: a New Biocatalyst With Broad-Spectrum L-Specific Amidase Activity”, Appl Microbial Biotechnol, vol. 39, No. 3, XP-008006772, 1993, pp. 296-300.
Akiyama Takanori
Katoh Osamu
Nakamura Tetsuji
Mitsubishi Rayon Co. Ltd.
Oblon, Spivak McClelland, Maier & Neustadt, L.L.P.
Steadman David J
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