Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2006-08-17
2009-06-30
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S183000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07553650
ABSTRACT:
The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived fromStreptoverticillium mobaraense(MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
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Hirayama Kazuo
Ishikawa Kohki
Kashiwagi Tatsuki
Shimba Nobuhisa
Suzuki Ei-ichiro
Ajinomoto Co. Inc.
Fronda Christian L
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
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