Polynucleotide constructs encoding aspartate kinase,...

Details Polynucleotide constructs encoding aspartate kinase,... Polynucleotide constructs encoding aspartate kinase,...

2008-05-06

2008-05-06

Steadman, David J. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S320100, C536S023200, C536S023700, C536S023100

Reexamination Certificate

active

07368276

ABSTRACT:
The invention relates to production of lysine, and provides several isolated polynucleotide molecules useful for the production of L-lysine. One such polynucleotide encodes an aspartate kinase (ask), an aspartate-semialdehyde dehydrogenase (asd) and a dihydrodipicolinate reductase. Other polypeptides encode ask, asd, dihydrodipicolinate reductase, and a diaminopimelate dehydrogenase (ddh); ask, asd, dihydrodipicolinate reductase, ddh, and an ORF2 polypeptide; and ask, asd, dihydrodipicolinate reductase, ddh, ORF2 and a diaminopimelate decarboxylase. The invention further provides methods of making and using the polynucleotides, and methods to increase the production of L-lysine. The invention further provides use of isolated polynucleotide molecules encoded by genes native to bacteria of the genusCorynebacterium. The invention further provides host cells bearing the isolated polynucleotide molecules of the invention. The invention further provides embodiments in which the host cell is a member of the genusCorynebacterium.

REFERENCES:
patent: 4346170 (1982-08-01), Sano et al.
patent: 4411997 (1983-10-01), Shimazaki et al.
patent: 4514502 (1985-04-01), Miwa et al.
patent: 4559308 (1985-12-01), Nutter et al.
patent: 4601983 (1986-07-01), Nakamori et al.
patent: 4710471 (1987-12-01), Katsumata et al.
patent: 4757009 (1988-07-01), Sano et al.
patent: 4778762 (1988-10-01), Miwa et al.
patent: 4822738 (1989-04-01), Miwa et al.
patent: 4861722 (1989-08-01), Sano et al.
patent: 4954441 (1990-09-01), Katsumata et al.
patent: 4980285 (1990-12-01), Sano et al.
patent: 5158891 (1992-10-01), Takeda et al.
patent: 5236831 (1993-08-01), Katsumata et al.
patent: 5243039 (1993-09-01), Schendel et al.
patent: 5380657 (1995-01-01), Schaefer et al.
patent: 5426050 (1995-06-01), Morinaga et al.
patent: 5426052 (1995-06-01), Flickinger et al.
patent: 5547864 (1996-08-01), Kawasaki et al.
patent: 5591577 (1997-01-01), Tsuchiya et al.
patent: 5597727 (1997-01-01), Kohama et al.
patent: 5633154 (1997-05-01), Schaefer et al.
patent: 5643790 (1997-07-01), Morinaga et al.
patent: 5661012 (1997-08-01), Sano et al.
patent: 5688671 (1997-11-01), Sugimoto et al.
patent: 5693781 (1997-12-01), Zupancic et al.
patent: 5700661 (1997-12-01), Katsumata et al.
patent: 5726299 (1998-03-01), Zupancic et al.
patent: 5766925 (1998-06-01), Sugimoto et al.
patent: 5804414 (1998-09-01), Moriya et al.
patent: 5876983 (1999-03-01), Sugimoto et al.
patent: 5929221 (1999-07-01), Kimura et al.
patent: 5965391 (1999-10-01), Reinscheid et al.
patent: 5989875 (1999-11-01), Kojima et al.
patent: 6004773 (1999-12-01), Araki et al.
patent: 6027920 (2000-02-01), Joliff et al.
patent: 6037154 (2000-03-01), Suga et al.
patent: 6040160 (2000-03-01), Kojima et al.
patent: 6090597 (2000-07-01), Hirano et al.
patent: 6200785 (2001-03-01), Kreutzer et al.
patent: 6221636 (2001-04-01), Hayakawa et al.
patent: 0 387 527 (1990-09-01), None
patent: 0 733 710 (1996-09-01), None
patent: 0 754 756 (1997-01-01), None
patent: 0 841 395 (1998-05-01), None
patent: 0 854 189 (1998-07-01), None
patent: 0 857 784 (1998-08-01), None
patent: 1 108 790 (2001-06-01), None
patent: WO 96/34961 (1996-11-01), None
patent: WO 01/49854 (2001-07-01), None
“Introduction to Protein Structure”, Branden and Tooze, Garland Publishing Inc., New York, 1991, p. 247.
Witkowski et al. (1999) Biochemistry 38:11643-11650.
Guo et al. Proc Natl Acad Sci 101:9205-9210, 2004.
Hult et al Curr Opin Biotechnol 14:395-400, 2003.
Archer, J.A.C., and Sinskey, A.J., “The DNA sequence and minimal replicon of theCorynebacterium glutamicumplasmid pSR1: evidence of a common ancestry with plasmids fromC. diphtheriae,” J. Gen. Microbiol. 139:1753-1759, (1993).
Ben-Samoun, K., et al., “Positively regulated expression of theEscherichia coli araBADpromoter inCorynebacterium glutamicum,” FEMS Microbiol. Lett. 174:125-130, (1999).
Jobling, M.G., and Holmes, R.K., “Construction of vectors with the p15a replicon, kanamycin resistance, induciblelacZαand pUC18 or pUC19 multiple cloning site,”Nucl. Acids Res. 18:5315-5316, (1990).
Kleemann A., et al., “Amino Acids,” inUllman's Encyclopedia of Industrial Chemistry, Fifth, Completely Revised Edition, vol. A 2:Amines, Aliphatic to Antibiotics, Gerhartz, W., ed., VCH Verlagsgesellschaft mbH, pp. 57-97 (1985).
Lonsdale, D.M., et al., “pFC1 to pFC7: A Novel Family of Combinatorial Cloning Vectors,”Plant Mol. Biol. Report. 13:343-345, Transaction Periodicals Consortium, (1995).
Marcel, T., et al., “Nucleotide sequence and organization of the upstream region of theCorynebacterium glutamicum lysAgene,”Mol. Microbiol. 4:1819-1830, (1990).
Nakayama, K., et al., “Microbial Production of Essential Amino Acids withCorynebacterium glutamicumMutants,” inNutritional Improvement of Food and Feed Proteins, Freidman, M., ed., Plenum Press, New York, NY, pp. 649-661 (1977).
Oguiza, J.A., et al., “A Gene Encoding Arginyl-tRNA Synthetase Is Located in the Upstream Region of the lysA Gene inBrevibacterium lactofermentum: Regulation ofargS-lysACluster Expression by Arginine,”J. Bacteriol. 175:7356-7362, (1993).
Pátek, M., et al., “Promoters fromCorynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif,”Microbiology142:1297-1309, (1996).
Pátek, M., et al., “Identification and transcriptional analysis of thedapB-ORF2-dapA-ORF4 operon of theCorynebacterium glutamicum, encoding two enzymes involved in L-lysine synthesis,”Biotechnol. Lett. 19:1113-1117, (1997).
Pharmingen “pPMG-LIC Bacterial Cloning Vector,” in1999 Research Products Catalog, Pharmingen, San Diego, CA, p. 839 (1999).
Record, Jr., M.T., et al., “Escherichia coliRNA Polymerase (Eσ70), Promoters, and the Kinetics of the Steps of Transcription Initiation,” inEscherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition, vol. I, Neidhardt, F.C., et al., eds., ASM Press, Washington, DC, pp. 792-821 (1996).
Reinscheid, D.J., et al., “Cloning, sequence analysis, expression and inactivation of theCorynebacterium glutamicum pta-ackoperon encoding phosphotransacetylase and acetate kinase,”Microbiology 145:503-513, (1999).
Schäfer, A., et al., “Small mobilizable multi-purpose cloning vectors derived from theEscherichia coliplasmids pK18 and pK19: selection of defined deletions in the chromosome ofCorynebacterium glutamicum,” Gene 145:69-73, (1994).
Serebrijski, I., et al., “Multicopy Suppression byasdGene and Osmotic Stress-Dependent Complementation by HeterologousproAMutants,”J. Bacteriol. 177:7255-7260, (1995).
Serwold-Davis, T.M., et al., “Transformation ofCorynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, andEscherichia coliwith theC. diphtheriaeplasmid pNG2,”Proc. Natl. Acad. Sci. USA 84:4964-4968, (1987).
Van Walsem, H.J., and Thompson, M.C., “Simulated moving bed in the production of lysine,”J. Biotechnol. 59:127-132, (1997).
Wendisch, V. F., et al., “Regulation of acetate metabolism inCorynebacterium glutamicum: transcriptional control of the isocitrate lyase and malate synthase genes,”Arch. Microbiol. 168:262-269, (1997).
Database EMBL Acc. No. Z21502, Pisabarro, A., et al. (Aug. 16, 1993).
Database EMBL Acc. No. AX122246, Nakagawa, S., et al. (May 11, 2001).
Database EMBL Acc. No. AX123535, Nakagawa, S., et al. (May 11, 2001).
Database EMBL Acc. No. AX125746, Nakagawa, S., et al. (May 10, 2001).
Anonymous, “Advances in the Biotechnology of Lysine Production,”Nutr. Rev. 43:88-90, International Life Sciences Institute (1985).
Bannur, B.B., and Purandare, G.M., “Microbial Production of L-lysine,”Hind. Antibiot. Bull. 11:191-205, (1969).
Batt, C.A., et al., “Genetic engineering of coryneform bacteria,”Trends Biotechnol. 3:305-310, (1985).
Chatterjee, M., and Chatterjee, S.P., “Microbial Production of L-lysin

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