Polynucleotide capture assay employing in vitro amplification

Details Polynucleotide capture assay employing in vitro amplification Polynucleotide capture assay employing in vitro amplification

1990-03-23

1993-04-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435210, 435 91, 536 243, 536 2433, 935 77, 935 78, 436 94, C12Q 168, C07H 1512

Patent

active

052003140

ABSTRACT:
An analyte polynucleotide strand having an analyte sequence is detected within a sample containing polynucleotides by contacting the analyte polynucleotide with a capture probe under hybridization conditions, where the capture probe has a first binding partner specific for a solid-phase second binding partner. The resulting duplex is then immobilized by specific binding between the binding partners, and non-bound polynucleotides are separated from the bound species. The analyte polynucleotide is optionally displaced from the solid phase, then amplified by PCR. The PCR primers each have a polynucleotide region capable of hybridizing to a region of the analyte polynucleotide, and at least one of the primers further has an additional binding partner capable of binding a solid-phase binding partner. The amplified product is then separated from the reaction mixture by specific binding between the binding partners, and the amplified product is detected.

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