Polynucleotide arrays

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Details

C536S024300, C536S024330, C536S025300, C435S006120, C435S091100

Reexamination Certificate

active

07553943

ABSTRACT:
The invention provides a hairpin polynucleotide, having a loop and a stem region, characterised in that a sulfur-based nucleophile is attached to an internal nucleotide in the hairpin through a linker to enable binding to a solid support.

REFERENCES:
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patent: WO 02/061126 (2002-08-01), None
Zhao et al., Immobilization of oligodeoxyribonucleotides with multiple anchors to microchips, Nucleic Acids Research, 29:955-959 (2001).
Broude, Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology, Trends in Biotechnology, 20:249-256 (2002).
Fang et al., Designing a Novel Molecular Beacon for Surface-Immobilized DNA Hybridization Studies, J. Am. Chem. Soc., 121:2921-2922 (1999).
Pirrung et al., Novel Reagents and Procedures for Immobilization of DNA on Glass Microchips for Primer Extension, Langmuir; 16:2185-2191 (2000).
Riccelli et al., Hybridzation of single-stranded DNA targets to immobilized complementary DNA probes: comparison of hairpin versus linear capture probes, Nucleic Acids Research, 29:996-1004 (2001).

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