Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-03
1997-08-12
Elliott, George G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 324260, 73 5418, C12Q 168, C12P 1934, G01N 1100, G01R 3300
Patent
active
056564295
ABSTRACT:
A method for sequencing nucleotides by attaching magnetizable moieties to the components of a mixture of such polynucleotide proteins and fragments, subjecting the mixture to a separation procedure to distribute the compounds on a substrate in a pattern according to molecular size and quantity, imparting magnetic properties to the attached magnetizable moieties, and then subjecting the substrate to magnetic reading to determine the molecular size and quantity of the compounds. Also disclosed is a method for monitoring a molecule amplification process wherein primers are used to produce copies of a molecule, which primers become part of the copy of the molecule by attaching a magnetized moiety to each primer such that the copy has magnetizable moieties at each end. The magnetizable moieties may then be magnetized. By subjecting the mixture to a magnetic field, the primers in the mixture can be separated from the copies produced and then the copies produced can be analyzed by a magnetic field sensor to determine the amount of copies produced.
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Elliott George G.
Fredman Jeffrey
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