Polymers of oligonucleotide probes as the bound ligands for use

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, C12Q 168, C12P 1934

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active

056838727

ABSTRACT:
A method of detecting nucleic acid sequences in which polymers of selected oligonucleotide probes which are complementary to a region in a nucleic acid sequence that is to be detected are bound to a substrate. The polymers bound to the substrate contain multiple randomly repeated copies of a specific oligonucleotide probe and may be synthesized using enzymatic amplification techniques.

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Mullis, Cold Spring Harbor Symp. 263-273 (1986) "Specific enzymatic amplification . . . " USB Catalogue (1990) p. 152.
Roewer et al. Electrophoresis: 12(2-3):181-186 (1991) "Hybridization and PCR . . . ".
Saiki et al. PNAS 86:6230-6234 (1989) "Genetic analysis of amplified DNA . . . ".
Murray et al., Mol. Biochem. Porasit. 30:209-216 (1988) "Cloning and characterization of a species . . . ".
Matthews et al., Analyt. Biochem 169:1-25 (1988) "Analytical strategies for the use of DNA probes".

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