Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1994-12-06
1996-10-22
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204454, C07K 126, C25B 700, C25B 1500
Patent
active
055672922
ABSTRACT:
The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20.degree. C. to about 50.degree. C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5.times.10.sup.3 to about 1.times.10.sup.6 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.
REFERENCES:
patent: 4963243 (1990-02-01), Ogawa et al.
patent: 5069766 (1991-12-01), Zhu et al.
patent: 5164055 (1992-11-01), Dubrow
patent: 5264101 (1992-05-01), Demorest et al.
Karger et al. Nucleic Acids Research 19(18):4955-4962 (1991) (no month) Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis.
Smith et al. Methods in Enzymology vol. 155 pp. 260-301, Academic Press (1991). Methods in Enzymology 155:260-301, Academic Press (1991) (no month) The synthesis and use of fluorescent oligonucleotides in DNA sequence analysis.
Grant et al., Chemical Dictionary, 5th ed., p. 122.
Efcavitch J. William
Grossman Paul D.
Madabhushi Ramakrishna S.
Menchen Steven M.
Grossman Paul D.
Niebling John
The Perkin-Elmer Corporation
Wong Edna
LandOfFree
Polymers for separation of biomolecules by capillary electrophor does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polymers for separation of biomolecules by capillary electrophor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polymers for separation of biomolecules by capillary electrophor will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2355753