Polymerization-induced separation immunoassays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 6, 436501, 436504, 436548, 436539, 436548, 525904, 5262381, 527202, G01N 33537

Patent

active

048430102

ABSTRACT:
Immunoassay methods and compositions are disclosed for the detection of analytes in fluid samples. The disclosure provides conjugates of analytes or reactants with polymerizable organic monomers. Specific binding reactions between reactants are detected by means of resporter/reactant conjugates. Free and specifically-bound reporter/reactant conjugates are separated by a polymerization reaction which renders the polymerized monomers insolule.

REFERENCES:
patent: 3969287 (1976-07-01), Jaworek
patent: 4469796 (1984-09-01), Axen
patent: 4474892 (1984-10-01), Murad
patent: 4609707 (1986-09-01), Nowinski

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