Polymeric sorbent sheets as ion reservoirs for electroblotting

Details Polymeric sorbent sheets as ion reservoirs for electroblotting Polymeric sorbent sheets as ion reservoirs for electroblotting

2007-12-13

2011-12-13

Barton, Jeffrey T (Department: 1759)

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

C204S613000, C204S464000

Reexamination Certificate

active

08075755

ABSTRACT:
Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.

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