Polymeric reagents for immobilizing biopolymers

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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Details

435 71, 525 50, 525539, 436518, C12Q 168, C08G 6348, G01N 33543, C07H 1900

Patent

active

061106690

ABSTRACT:
Reagents for the immobilization of biopolymers, processes for their preparation and their subsequent use in the immobilized of biopolymers for analytical and diagnostic procedures are described. One type of reagent includes a solid support fabricated of a polymeric material having at least one surface with pendant acyl fluoride functionalities, Another reagent includes solid supports fabricated of polymeric materials including ethylene acrylic acid or ethylene methacrylic acid copolymers and activated polypropylene. Processes for preparing reagents include derivatizing polymeric materials to form acyl fluoride functionalities or derivatizing ethylene acrylic acid copolymers and ethylene methacrylic acid copolymers to form active acyl functionalities. Processes for immobilizing biopolymers include attaching natural or presynthesized biopolymers to activated solid support surfaces and directly attaching in a step-wise successive manner biomonomer units to a growing biopolymer chain attached to the solid support reagent.

REFERENCES:
patent: 5424186 (1995-06-01), Fodor et al.

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