Polymeric electrode for electrophysiological testing

Details Polymeric electrode for electrophysiological testing Polymeric electrode for electrophysiological testing

2005-05-31

2005-05-31

Noguerola, Alex (Department: 1753)

Chemistry: electrical and wave energy

Apparatus

Electrolytic

C204S297100, C435S173400, C435S287100

Reexamination Certificate

active

06899800

ABSTRACT:
A polymeric material such as PDMS is molded into an electrode structure containing a micron-size aperture for receiving and forming a giga-ohm seal with a biological membrane. One end of a tube is filled with uncured polymeric material and pressed against a support surface to prevent drainage. A conventional micropipette having a size suitable for sliding through the tube is introduced, tip first, into the tube and is allowed to fall through the polymeric material and rest against the support surface. The assembly is heated to cure the polymer and the micropipette is removed from the tube, thereby leaving a polymeric plug at the end of the tube with an aperture suitable in shape and size for patch-clamp giga-ohm seal electrode applications. A multi-well tray with a polymeric electrode plug in each well is constructed using the same approach.

REFERENCES:
patent: 5799151 (1998-08-01), Hoffer
patent: 5823428 (1998-10-01), Humberstone et al.
patent: 6315940 (2001-11-01), Nisch et al.
patent: WO 01/25769 (2001-04-01), None
Kostyuk et al. (“Effect of internal fluoride and phosphate on membrane currents during intracellular dialysis of nerve cells,” Nature vol. 257, Oct. 23, 1975).

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