Polymerase enhancing factor (PEF) extracts, PEF protein...

Details Polymerase enhancing factor (PEF) extracts, PEF protein... Polymerase enhancing factor (PEF) extracts, PEF protein...

2000-08-04

2009-12-01

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

Reexamination Certificate

active

07625725

ABSTRACT:
The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information forPyrococcus furioususPEF (P45), which was used to produce a recombinant PEF.

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