Polymerase compositions and uses thereof

Details Polymerase compositions and uses thereof Polymerase compositions and uses thereof

2007-12-25

2007-12-25

Fredman, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C536S024300

Reexamination Certificate

active

08529767

ABSTRACT:
The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3′–5′ exonuclease activity (b) a DNA polymerase with less 3′–5′ exonuclease activity than the enzyme with substantial 3′–5′ exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3′–5′ exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase (isolated fromThermus aquaticus) and the Pfu DNA polymerase (isolated fromPyrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides, typically DNA, using compositions comprising an enzyme that possesses substantial 3′–5′ exonuclease activity and a DNA polymerase with less 3′–5′ exonuclease activity than the enzymes possessing substantial 3′–5′ exonuclease activity, preferably a DNA polymerase that substantially lacks 3′–5′ exonuclease activity. Another aspect of the invention involves the use the subject method of polynucleotide synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of polynucleotides, wherein the kits comprise an enzyme that possesses substantial 3′–5′ exonuclease activity and a DNA polymerase with less 3′–5′ exonuclease activity than the enzyme possessing substantial 3′–5′ exonuclease activity.

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Summons, undated.
Summons in a Civil Case, undated.
Summons in a Civil Action, undated.
Plaintiff Takara Bio, Inc.'s First Amended Complaint Under the Patent Law, undated.
Notice of Motion to Dismiss, Transfer or Stay Action, with Exhibits A-V, undated.
Takara Bio, Inc.'s Memorandum of Points and Authorities in Opposition to Strategene's Motion to Dismiss, Stay or Transfer, with Declaration of John B. Pegram and Exhibits 1-4, 6, 8, 9, and 11-32, undated.
Defendant Stratagene's Reply in Support of Motion to Transfer, Dismiss or Stay, with Exhibits A and B, undated.
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Letter Concerning Request by Takara Bio Inc. for Leave to Participate in this Reexamination as a Partial Assignee (Co-owner) and other Takara Bio Inc. Submissions, with Exhibits 1 and 2, undated.
Letter Submitting Copy of Request by Takara Bio Inc. for Leave to Participate in this Reexamination as a Partial Assignee (Co-owner) and other Takara Bio Inc. Submissions, undated.
Reply to Owner's Statement Under 37 C.F.R. § 1.535, Transmittal Form, and Certificate of Service, undated.
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Complaint for Patent Infringement.
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