Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2000-12-29
2004-03-02
Whisendnt, Ethan (Department: 1637)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S303100, C435S285100, C356S417000
Reexamination Certificate
active
06699713
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to instruments for chemical reaction, amplification, and detection, and in particular, to a system for doing polymerase chain reaction, amplification, and detection.
2. State of Technology
U. S. Pat. No. 5,589,136 for silicon-based sleeve devices for chemical reactions, by Northrup et al, patented Dec. 31, 1996, provides the following description: “A silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.”
A paper titled “Handheld advanced Nucleic Acid Analyzer” was presented by W. J. Benett, J. B. Richards, P. Stratton, D. R. Hadley, B. H. Bodtker, S. L. Nasarabadi, F. P. Milanovich, R. P. Mariella, R. P. Koopman, and P. Belgrader at the Society of Photo-Optical Instrumentation Engineers Symposium on Environmental and Industrial Sensing, Boston, Mass. Nov. 5-8, 2000. This paper is incorporated herein by reference.
A paper titled “Miniaturized detection system for handheld PCR assays” was presented by J. B. Richards, W. J. Benett, P. Stratton, D. R. Hadley, S. L. Nasarabadi, and F. P. Milanovich, at the Society of Photo-Optical Instrumentation Engineers Symposium on Environmental and Industrial Sensing, Boston, Mass. Nov. 5-8, 2000. This paper is incorporated herein by reference.
SUMMARY OF THE INVENTION
The present invention provides a polymerase chain reaction DNA amplification and detection system. An embodiment of the system includes one or more sample chamber modules adapted to contain biological sample volumes. Each of the sample chamber modules has the ability to support a duplex assay and has parallel interrogation ports operatively connected to the chamber modules. Each of the ports has an optical fluorescence detection system. Additional aspects, advantages, and features of the invention are set forth in part in the following description. Various aspects, advantages, and features of the invention will become apparent to those skilled in the art upon examination of the description and by practice of the invention.
REFERENCES:
patent: 5589136 (1996-12-01), Northrup et al.
patent: 5721123 (1998-02-01), Hayes et al.
patent: 5965410 (1999-10-01), Chow et al.
patent: 6369893 (2002-04-01), Christel et al.
patent: WO98/43740 (1998-10-01), None
patent: WO99/12016 (1999-03-01), None
patent: WO99/48608 (1999-09-01), None
W.J. Benett, et al., “Handheld Advanced Nucleic Acid Analyzer,” UCRL-JC-136587, Preprint, U.S. Dept. of Energy, Lawrence Livermore National Laboratory, Oct. 6, 2000, 11 pages.
James B. Richards, et al., “Miniaturized Detection System for Handheld PCR Assays,” UCRL-JC-138399 Preprint, U.S. Dept. of Energy, Lawrence Livermore National Laboratory, Oct. 6, 2000, 12 pages.
Belgrader Phil
Benett William J.
Hadley Dean R.
Meyer Peter L.
Milanovich Fred P.
Hashemi Shar
Scott Eddie E.
The Regents of the University of California
Thompson Alan H.
Whisendnt Ethan
LandOfFree
Polymerase chain reaction system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polymerase chain reaction system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polymerase chain reaction system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3187737