Polymerase chain reaction-restriction fragment length polymorphi

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 435 911, 536 231, 536 2433, 935 31, 935 8, 935 27, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500

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058769773

ABSTRACT:
This invention provides a procedure for authentication of plant and animal materials used as traditional Chinese medicine is described. This method amplifies and detects the discrete and species-specific RFLP patterns in the region of rDNA. The present invention offers a reliable and definite way to identify morphologically similar Chinese medicine, using a minute amount of biological samples. Its application in the authentication of American and Oriental ginsengs is illustrated in detail.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
David M. Hillis and Michael T. Dixon, The Quarterly Review of Biology vol. 66 No. 4: 410-449, (1991).
Wen-Sheng Lang, et al., Journal of Chinese Pharmaceutical Sciences, pp. 133-143, (1993).
Jun Wen, et al., Molecular Phylogenetics And Evolution vol. 6, No. 2, pp. 167-177, Article No. 0069, (1996).

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