Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-11-01
1999-01-26
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 4352523, 4353201, 536 2432, 536 2433, 536 253, C12Q 168, C12P 1934, C07H 2104
Patent
active
058637311
ABSTRACT:
This invention provides methods and reagents for screening plants, particularly geraniums, for infections with bacterial pathogens. Specifically, the invention provides nucleic acid amplification and detection methods for specifically detecting a certain pathovar of a bacterial infectious agent in geraniums. The invention provides a nucleic acid hybridization probe that is specific for Xanthomonas campestris pathovar pelargonii, and paired sets of oligonucleotide primers for polymerase chain reaction-catalyzed amplification of DNA fragments comprising the nucleic acid sequences of the invention specific for Xanthomonas campestris pathovar pelargonii template DNA. The invention also provides PCR-based methods for detecting Xanthomonas campestris pathovar pelargonii DNA, and in particular for distinguishing pathovar DNA from the DNA of the host infected plant. Methods for producing detectably-labeled hybridization probes using PCR are also provided.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis et al.
patent: 5411941 (1995-05-01), Grinna et al.
patent: 5420009 (1995-05-01), Hartung et al.
patent: 5427932 (1995-06-01), Weier et al.
Knauss and Tammen, (1967) Phytopathology 57:1178-1181.
Lazo et al., (1987) Int. J. Syst. Bacteriol. 37: 214-221.
Benedict et al., (1990) Appl. Environ. Microbial 56: 572-574.
Anderson & Nameth, (1990) Phytopathology 80: 357-360.
Vauterin et al., (1990) Syst. Appl. Microbiol. 13: 166-176.
Vauterin et al., (1991) Gen. Microbiol. 137: 1677-1687.
Rasmussen & Reeves, (1992) J. Biotechnol. 25: 203-220.
Seal et al. (1992) Appl. Environ. Microbiol. 58: 3751-3758.
Manulis et al., (1992) Phytoparasitica 20: 263.
Hartung et al., (1993) Appl. Environ. Microbiol. 59: 1143-1148.
Judd et al. (1993) Appl. Environ. Microbiol. 59: 1702-1708.
Manulis et al. (1994) Appl. Environ. Microbiol. 60: 4094-4099.
Sulzinski et al., (1995) J. Phytopathology 143: 429-433.
Versalovic et al., (1991) Nucleic Acids. Res. 19: 6823-6831.
Tuinier et al., (1989), Plant Diseases, 73: 875-878.
Hartung et al., (1993), Plant Diseases, 76: 889-893.
Sulzinski et al., J. Phytopathol. 144, 393-398 (1996).
Sulzinski et al., J. Phytopathol. 145, 213-215 (1997).
Moorman Gary W.
Romaine C. Peter
Sulzinski Michael A.
Horlick Kenneth R.
Monahan Thomas J.
The Penn State Research Foundation
LandOfFree
Polymerase chain reaction-based diagnostic test for the detectio does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polymerase chain reaction-based diagnostic test for the detectio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polymerase chain reaction-based diagnostic test for the detectio will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1449476