Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-10-14
1999-10-19
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
059687452
ABSTRACT:
A polymer-electrode including (a) a substrate having a conductive working surface; and (b) a polymer layer on the conductive working surface. The polymer layer has a plurality of microfluidic reaction openings distributed throughout the layer. An oligonucleotide probe can be attached to the polymer layer and is available to capture target nucleic acid. A soluble mediator can diffuse freely and transfer electrons from the preselected base in the hybridized nucleic acid to the conductive working surface of the substrate. An electronic signal generated from the electron transfer reaction is detected and quantitated.
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Loomis Carson R.
Napier Mary E.
Thorp H. Holden
Campbell Eggerton A.
The University of North Carolina at Chapel Hill
Xanthon, Inc.
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