Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-08-10
1997-01-21
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 6, 435 691, 435183, 435189, 435193, 435194, 435207, 4352402, 4353201, 530300, 530345, 530350, 530402, C07K 100, C07K 1107, C12N 1500, C12N 1588
Patent
active
055958974
ABSTRACT:
The invention concerns a complex between at least one negatively charged nucleic acid and at least one positively charged polymeric conjugate, the link between the nucleic acid and the polymeric conjugate being electrostatic in nature, the polymeric conjugate containing a polymer formed from monomer components having free NH.sub.3.sup.+ functions of the aforementioned components and being as follows:
--the free NH.sub.3.sup.+ functions from the aforementioned components are substituted in a ratio of at least 10%, advantageously from 45% to 70%, particularly 60%, by noncharged residues leading to a reduction of positive charges in comparison to the same nonsubstituted polymeric conjugate, facilitating the release of nucleic acid by the dissociation of the complex,
--the aforementioned residues possess in addition the following properties:
REFERENCES:
Crystal "Transfer of Genes to Humans: Early Lessons and Obstacles" Science 270:404-410 Oct. 1995.
Gura "Antisense has Growing Pains" Science 270:575-577 Oct. 1995.
Kabanov et al. "DNA Complexes with Polycations for the Delivery of Genetic Material into Cells" Bioconjugate Chem. 6:7-20 1995.
Erbacher Patrick
Midoux Patrick
Monsigny Michel
Roche-Degremont Annie-Claude
I.D.M. Immuno-Designed Molecules
Larson Thomas G.
LeGuyader John L.
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