Polyketides and their synthesis and use

Details Polyketides and their synthesis and use Polyketides and their synthesis and use

2006-03-28

2006-03-28

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

C435S075000, C435S117000, C435S183000, C435S252350, C536S023200

Reexamination Certificate

active

07018808

ABSTRACT:
The rapamycin gene cluster is an example of a gene cluster which includes a gene (rapL) leading to the formation of a precursor compound (pipecolic acid, in this case) which is required for inclusion in the larger product (rapamycin) produced by the enzymes encoded by the cluster. We have produced a mutant strain containing a rapamycin gene cluster in which the rapL gene is disabled. The strain does not naturally produce rapamycin but does so if fed with pipecolic acid. By feeding with alternative carboxylic acids we have produced variants of rapamycins. Tests have shown biological activity.

REFERENCES:
patent: 0589703 (1994-03-01), None
patent: WO 9410843 (1994-05-01), None
McDaniel, et al., “Engineered Biosynthesis of Novel Polyketides”, Science, 262:1546-1550, (1993).
C. D. Denoya et al., “A Second Branched-Chain α-Keto Acid Dehydrogenase Gene Cluster (bkdFGH) fromStreptomyces avermitilis: Its Relationship to Averxmectin Biosynthesis and the Construction of abkdFMutant Suitable fox the Production of Novel Antiparasitic Avermectins”, Journal of Bacteriology, 77(12) : 3504-3511 (1995).
E. Hafner et al., “Branched-Chain Fatty Acid Requirement for Avermectin Production by a Mutant ofStreptomyces avermitilisLacking Branched-Chain 2-Oxo Acid Dehydrogenase Activity”, The Journal of Antibiotics, 44(3) : 349-356 (1991).
Li Tang et al., “Amino Acid Catabolism and Antibiotic Synthesis: Valine is a Source of Precursors for Macrolide Biosynthesis inStreptomyces ambofaciens and Streptomyces fradiec”, Journal of Bacteriology, 176(19) : 6107-6119 (1994).
C. J. Dutton et al., “Novel Avermectins Produced by Mutational Biosynthesis”, 44(3) : 357-365 (1991).
N. Lomovskaya et al., “Gene disruption and replacement in the rapamycin-producingStreptomyces hygroscopicusstrain ATCC 29253”, Microbiology, 143: 875-883 (1997).
I. Molnar et al., “Organisation of the biosynthetic gene cluster for rapamycin inStreptomyces hygroscopicus: analysis of genes flanking the polyketide synthase”, Gene, 169: 1-7 (1996).
T. Schwecke et al. “The biosynthetic gene cluster for the polyketide immunosuppressant repamycin”, Proc. Natl. Acad. Sci. USA, 92: 7839-7843 (1995).
L. L. Khaw et al., “Mutational Biosynthesis of Novel Rapamycins by a Strain ofStreptomyces hygroscopicusNRRL 5491 Disrupted in rapL, Encoding a Putative Lysine Cyclodeaminase”, Journal of Bacteriology, 180(4) : 809-814 (1998).

Affiliated with

Also associated with

Rating

Polyketides and their synthesis and use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polyketides and their synthesis and use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polyketides and their synthesis and use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3592019