Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1997-05-16
2000-05-09
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 32, 514 29, 536 72, C12Q 100, C12Q 118, C07H 1708
Patent
active
060602341
ABSTRACT:
The invention provides novel erythromycin derivatives in which methyl groups on the macrolactone ring have been substituted with --H, --Et, and/or --OH. The invention also provides reagents such as isolated polynucleotides, vectors comprising the polynucleotides and host cells transformed with the vectors for making the novel compounds. Methods for making the compounds utilizing genetic engineering techniques are also disclosed.
REFERENCES:
patent: 3869445 (1975-03-01), Hallas et al.
patent: 3884902 (1975-05-01), Hallas et al.
patent: 4740502 (1988-04-01), Hannick et al.
patent: 4742049 (1988-05-01), Baker et al.
patent: 4833236 (1989-05-01), Morimoto et al.
patent: 4857641 (1989-08-01), Hauske
patent: 4874748 (1989-10-01), Katz et al.
patent: 4921801 (1990-05-01), Rao et al.
patent: 4935340 (1990-06-01), Baltz et al.
patent: 5081023 (1992-01-01), Yaginuma et al.
patent: 5087563 (1992-02-01), Beremand et al.
patent: 5110728 (1992-05-01), Kridl et al.
patent: 5141926 (1992-08-01), Weber et al.
patent: 5252474 (1993-10-01), Gewain et al.
patent: 5672491 (1997-09-01), Khosla et al.
patent: 5712146 (1998-01-01), Khosla et al.
patent: 5744350 (1998-04-01), Vinci et al.
patent: 5801032 (1998-09-01), Stassi et al.
Schwecke, T., et al., Proceedings of the National Academy of Sciences, U.S.A., vol. 92, "The Biosynthetic Gene Cluster for the Polyketide Immunosuppressant Rapamycin", pp. 7839-7843 (1995).
Han, L. et al., Microbiology, vol. 140, "Cloning and Characterization of Polyketide Synthase Genes for Jadomycin B Biosynthesis in Streptomyces Venezuelae ISP5230", pp. 3379-3389 (1994).
MacNeil, D.J., et al., Annals of the New York Academy of Sciences, vol. 721, "Correlation of the Avermectin Polyketide Synthase Genes to the Avermectin Structure", pp. 123-132 (1994).
Hu, Z., et al., Molecular Microbiology, vol. 14, "Repeated Polyketide Synthase Modules Involved in the Biosynthesis of a Heptaene Macrolide by Streptomyces sp. FR-008", pp. 163-172 (1994).
Fernandez-Moreno, M.A., et al., The Journal of Biological Chemistry, vol. 269, "DNA Sequence and Functions of the actVI Region of the Actinorhodin Biosynthetic Gene Cluster of Streptomyces Coelicolor A3(2)*", pp. 24854-24863 (1994).
Kim, E.S., et al., Journal of Bacteriology, vol. 177, "Heterologous Expression of an Engineered Biosynthetic Pathway: Functional Dissection of Type II Polyketide Synthase Components in Streptomyces Species", pp. 1202-1207 (1994).
Brown, M.J.B., et al., Journal of the Chemical Society, Chemical Communications, vol. 15, "A Mutant Generated by Expression of an Engineered DEBS1 Protein from the Erythromycin-Producing Polyketide Synthase in Streptomyce Coelicolor Produces the Triket" (1995).
Kao, C.M., et al., Journal of the American Chemical Society, vol. 117, "Manipulation of Macrolide Ring Size by Directed Muutagenesis of a Modular Polyketide Synthase", pp. 9105-9106 (1996).
Kuhstoss, S., et al., Gene, vol. 183, "Production of a Novel Polyketide Through the Construction of a Hybrid Polyketide Synthase", pp. 231-236 (1996).
Kao, C.M., et al., Biochemistry, vol. 35, "Evidence for Two Catalytically Independent Clusters of Active Sites in a Functional Modular Polyketide Synthase", pp. 12363-12368 (1996).
Aigle, B., et al., Microbiology, vol. 142, "An Amplifiable and Deletable Locus of Streptomyces RP18110 Contains a Very Large Gene Homologous to Polyketide Synthase Genes", pp. 2815-2824 (1996).
Aparicio, et al., "Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase", Gene, vol. 169 (1996), pp. 9-16.
Aparicio, et al., "Limited Proteolysis and Active-site Studies of the First Multienzyme Component of the Erythromycin-producing Polyketide Synthase", The Journal of Biological Chemistry, vol. 269 (1994), pp. 8524-8528.
Ashworth, et al., "On the biosynthetic origins of the hydrogen atoms in the macrotetrolide antibiotics: and their mode of assembly catalysed by a nonactin polyketide synthase", Journal of the Chemical Society, Perkin Transactions, vol. 1 (1989), p. 1461.
Baltz, et al. "Genetics of streptomyces fradiae and tylosin biosynthesis", Annual Review of Microbiology, vol. 42 (1988), pp. 547 & 558-574.
Bibb, et al., "Analysis of the Nucleotide sequence of the streptomyces glaucescens tcml genes provides key information about the enzymology of polyketide antibiotic biosynthesis", The EMBO Journal, vol. 8 (1989), pp. 2727-2736.
Caffrey, et al, "Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea", FEBS Letters, vol. 304 (1992), pp. 225, 228.
Cane, et al., "Macrolide Biosynthesis. 4. Intact Incorporation of a Chain-Elongation Intermediate into Erythromycin", J. Am. Chem. Soc., vol. 109 (1987), pp. 1255-1257.
Cortes, et al., "An unusually large multifunctional polypeptide in the erythromycin producing polyketide synthase of saccharopolyspora erythraea", Nature, vol. 348 (1990), pp. 176-178.
Cortes, et al., "Repositioning of a Domain in a Modular Polyketide Synthase to Promote Specific Chain Cleavage", Science, vol. 268 (1995), pp. 1487-1490.
Dhillon, et al., "Molecular characterization of a gene from saccharopolyspora-erythraea (streptomyces-erythraeus) which is involved in erythromycin biosynthesis", Molecular Microbiology, vol. 3 (1989), pp. 1405-1414.
Donadio, et al., "Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora etythraea", Gene, vol. 111 (1992), pp. 51-60.
Donadio, et al., "Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides" Gene, vol. 115 (1992), pp. 97-103.
Donadio, et al., "Modular Organization of Genes Required for Complex Polyketide Biosynthesis" Science, vol. 252 (1991), pp. 675-679.
Donadio, et al., "Genetic studies on erythromycin biosynthesis in saccharopolyspora erythraea", Genetics and Molecular Biology of Industrial Microorganism, (1989), pp. 53-59.
Harris, et al., "Initial planning", Protein Purification Methods: A Practical Approach, IRL Press, New York, NY; pp. 57-67.
Haydock, et al., "Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases", FEBS Letters, vol. 374 (1995), pp. 246-248.
Haydock, et al., "Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases", Mol. Gen. Genet., vol. 230 (1991), pp. 120-128.
Hopwood, et al., "`Hybrid` pathways for the production of secondary metabolites", GIM 90: Proceedings of the 6th International Symposium on Genetics of Industrial Microorganisms, vol. 1, pp. 259-270 (1991).
Hopwood, et al., "Molecular Genetics of Polyketides and its comparison to Fatty Acid Biosynthesis", Annu. Rev. Genet., vol. 24 (1990), pp. 37-66.
Hopwood, et al., "Production of `Hybrid` Antibiotics by Genetic Engineering", Nature, vol. 314 (1985), pp. 642-644.
Huber, et al., "Branched-chain fatty acids produced by mutants of streptomyces fradiae, putative precursors of the lactone ring of tylosin", Antimicrobial Agents and Chemotherapy, vol. 34 (1990), pp. 1535-1541.
Hutchinson, et al., "Polyketide synthase gene manipulation: A structure-function approach in engineering Novel antibiotics", Annu. Rev. Microbio., vol. 49 (1995), pp. 201-238.
Kakinuma, et al., "Genetic studies of the biosynthesis of kalafungin, a benzoisochromanequinone antibiotic", Tetrahedron, vol. 47, pp. 6059-6068 (1991).
Kao, et al., "Engineered Biosynthesis of a Complete Macrolactone in a Heterologous Host", Science, vol. 265 (1994), pp. 509-512.
Kao, et al., "Engineered Biosynthesis of a Triketide Lactone from an Incomplete Modular Polyketide Synthase" J. Am. Chem. Soc., vol. 116 (1994), pp. 11612-11613.
Kinoshita, et al., "Isolation of proposed intermediates in the biosynthesis of mycinamicins", J. Chem. Soc. Chem.
Kakavas Stephan J.
Katz Leonard
Pereda-Lopez Ana
Ruan Xiaoan
Stassi Diane L.
Abbott Laboratories
Casuto Dianne
Leary Louise N.
LandOfFree
Polyketide derivatives and recombinant methods for making same does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polyketide derivatives and recombinant methods for making same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polyketide derivatives and recombinant methods for making same will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1063607