Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Multi-enzyme system
Reexamination Certificate
2006-09-19
2006-09-19
Patterson, Jr., Charles L. (Department: 1652)
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Multi-enzyme system
Reexamination Certificate
active
07109007
ABSTRACT:
A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) fromZoogloea ramigerastrain I-16-M,Alcaligenes eutrophus, Nocardia salmonicolur, andPsuedomonas olevaranswere identified or isolated and expressed in a non-PHB producing organism,E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.
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Peoples Oliver P.
Sinskey Anthony J.
Pabst Patent Group LLP
Patterson Jr. Charles L.
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