Polyhydroxybutyrate polymerase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C528S361000

Reexamination Certificate

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06881560

ABSTRACT:
A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerise or PHA polymerase) fromZooloea ramigerastrain I-16-M,Alcaligenes eutrophus, Nocardia salmonicolur, andPsuedomnas olevaranswere identified or isolated and expressed in a non-PHB producing organism,E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.

REFERENCES:
patent: 3275610 (1966-09-01), Coty
patent: 4477654 (1984-10-01), Holmes et al.
patent: 5229279 (1993-07-01), Peoples et al.
patent: 5245023 (1993-09-01), Peoples et al.
patent: 5250430 (1993-10-01), Peoples et al.
patent: 5512669 (1996-04-01), Peoples et al.
patent: 5534432 (1996-07-01), Peoples et al.
patent: 5661026 (1997-08-01), Peoples et al.
patent: 5663063 (1997-09-01), Peoples et al.
patent: 0 069 497 (1987-04-01), None
patent: 0 204 442 (1991-11-01), None
patent: WO 8900202 (1989-01-01), None
patent: WO 9219747 (1992-11-01), None
patent: WO 9302187 (1993-02-01), None
patent: WO 9302194 (1993-02-01), None
patent: WO 9411519 (1994-05-01), None
patent: WO 9423027 (1994-10-01), None
patent: WO 9505472 (1995-02-01), None
An, Binary Ti Vectors for Plant Transformation and Promoter Analysis,Methods in Enzymology, 153, 293-305 (1987).
Anderson, “Shotgun DNA Sequencing Using Cloned DNase 1-Generated Fragments,”Nucleic Acids, 9, 3015-3026 (1981).
Anderson, et al., “Occurrence, Metabolism, Metabolic Role, and Industrial Uses of Bacterial Polyhydroxyalkanoates,”Microbiological Reviews, 54(4), 450-472 (1990).
Bagdasarian, et al., “Activity of the Hybrid-trp-lac(tac) Promoter ofEscherichia coliinPseudomonas putida. Construction of Broad-Host-Range, Controlled-Expression Vectors,”Gene, 26, 273-282 (1983).
Berg, et al., “The Prokaryotic Transposable Element Tn5,”Bio/Technology, 1, 417-435 (1983).
Berk & Sharp, “Sizing and Mapping of Early Adenovirus mRNAs By Gel Electrophoresis of S1 Endonuclease-Digested,”Cell, 12, 721-732 (1977).
Berndt & Schlegel, “Kinetics and Properties of β-Ketothiolase fromClostridium pasteurianum,” Arch. Microbiol., 103, 21-30 (1975).
Bighee, et al., “Monoclonal Antibodies Specific for the M- and N-Forms of Human Glycophorin A*,”Mol. Immunol., 20, 1353-1357 (1983).
Birnboim & Doly, “A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA,” inNucleic Acids Res., 7, 1513-1523 (1979).
Bradford, “A Rapid and Senstive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Building,”Anal. Biochem., 72, 248-254 (1976).
Brandl, et al., “Pseudomonas oleovoransas a Source of Poly(β-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters,”App. Environ. Microbiol., 54, 1977-1982 (1988).
Burnette, “‘Wester Blotting’: Electrophoretic Transfer of Proteins from Sodium Dodecyl Sulfate-Polyacrylamide Gets to Unmodified Nitrocellulose and Radiographic Detection with Antibody and Radioiodinated Protein A,”Anal. Biochem., 112, 195-203 (1981).
Cashmore, “Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase,” 29-38.
Comai, et al., “Expression in Plants of a MutantaroAGene fromSalmonella typhimuriumconfers Tolerance to Glyphosate,”Nature, 317, 741-744 (1985).
Davis, et al., “Biosynthetic Thiolase fromZoogloea ramigera,” J. Biol. Chem., 262, 82-89 (1987).
De Block, et al., “Engineering Herbicide Reistance in Plants by Expression of a Detoxifying Enzyme,” 2513-2518 (1987).
De Smet, et al., “Characterization of Intracellular Inclusions Formed byPseudomonas oleovoransDuring Growth on Octane,”J. Bacteriol., 154(2), 870-878 (1983).
Ditta, et al., “Broad Host Range DNA Cloning System for Gram-Negative Bacteria: Construction of a Gene Bank of Rhiozobium Meliloti,”Proc. Natl. Acad. Sci. USA, 77(12), 7347-7351 (1980).
Easson, et al., “Isolation andZoogloea ramigeraI-16-M Exopolysaachride Biosynthetic Genes and Evidence for Instability Within this Region,”J. Bacteriol., 169, 4518-4524 (1987).
Everett, et al., “Genetic Engineering of Sunflower (Helinathus annuusL.),”Bio/Technology, 5, 1201-1204 (1987).
Firoozobady, et al., “Transformation of Cotton (Gossypium hirsutumL.) byAgrobacterium tumefaciensand Regeneraton of Transgenic Plants,”Plant Molecular Biology, 10, 105-116 (1987).
Fischhoff, et al., “Insect Tolerant Transgenic Tomato Plants,”Bio/Technology, 5, 807-813 (1987).
Friedman, et al., “Construction of a Broad Host Range Cosmid CloningVector and its Use in the Genetic Analysis of Rhizobium Mutants,”Gene, 18, 289-296 (1982).
Fromm, et al., Electroporation of DNA and RNA into Plant Protoplasts,Methods of Enzymology, 153, 351-366 (1987).
Fukui, et al., “Enzymatic Synthesis of Poly-β-Hydroxybutyrate inZoogloea ramigera,” Arch. Microbiol., 110, 149-156 (1976).
Fukui, et al., “Purification and Characterization of NADP-Linked Acetoacetyl-CoA Reductase fromZoogloea ramigeraI-16-M,”Biochem. Biophys. Acta, 917, 365-371 (1987).
Gill, et al., “Overproduction and Assay ofPseudomonas aeruginosaPhosphomannose Isomerase,”J. Bact., 167, 611-617 (1986).
Griebel & Merrick, “Metabolism of Poly-β-Hydroxybutyrate: Effect of Mile Alkaline Extraction on Native Poly-β-Hydroxybutyrate Granules,”J. Bacteriol., 108, 782-789 (1971).
Handbook of Plant Cell Culture, vol. 4, edited by D.A. Evans, W.R. Sharp, and P.V. Ammirate (Macmillan Publishing Co., NY 1986) (contents only).
Haywood, et al., “The Importance of PHB-Synthese Substrate Specificity in Polyhydroxyalkaneate synthesis byAlcaligenes eutrophus,” Chemical Abstracts, 110(15) p. 404, Abstract 131921p (1989).
Hiatt, et al., “Production of Antibodies in Transgenic Plants,”Nature, 342, 76-78 (1989).
Hinchee, et al., “Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer,”Bio/Technology, 915-922 (1988).
Hinnenbusch, et al., “Repeated DNA Sequences Upstream from HISI Also Occur at Several Other Co-Regulated Genes inSaccharomyces cerevisiae*,” J. Biol. Chem., 258, 5238-5247 (1983).
Hoekema, et al., “The Genetic Engineering of Two Commercial Potato Cultivars for Resistance to Potato Virus X,”Bio/Technology, 7, 273-278 (1989).
Hohn & Murray, “Packagin Recombinant DNA Molecules into Bacteriophage Particles in Vitro,”Proc. Natl. Acad. Sci., USA, 74, 3259-3263 (1977).
Hooykaas, et al., “Detection of Monocot Transformation viaAgrabacterium tumefaciens,” Methods in Enzymology, 153, 305-313 (1987).
Horsch, et al., “Inheritance of Functional Foreign Genes in Plants,”Science, 223, 496-498 (1983).
Ish-Horowicz & Burke, “Rapid and Efficient Cosmid Cloning,”Nucleic Acids Res., 9, 2989-2998 (1981).
Kleckner, et al., “Genetic Engineering in Vivio Using Translocatable Drug-Reistance Elements,”J. Mol. Biol., 116,125-159 (1977).
Laemmli, “Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4,”Nature, 222, 680-685 (1970).
Lageveen, et al., “Formation of Polyesters byPseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydr

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