Polyhedrin gene and genetic engineering thereof

Details Polyhedrin gene and genetic engineering thereof Polyhedrin gene and genetic engineering thereof

1990-02-23

1992-09-08

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4354123, 4353207, 4352401, 537 27, C12P 2102, C12N 1534, C12N 1586, C12N 1500

Patent

active

051457750

ABSTRACT:
A polyhedrin gene of Spodoptera litura nuclear polyhedrosis virus (SlNPV C-411) has the following restriction enzyme cleavage map and about 3 kilobase pairs: ##STR1## At least a part of said polyhedrin gene can be substituted by a gene coding for a useful substance to construct a vector and a recombinant virus, and it is possible to produce a useful substance such as a peptide, protein or glycoprotein by culturing a cell infected with the recombinant virus.

REFERENCES:
patent: 4745051 (1988-05-01), Smith et al.
Gonzalez et al., Virology 170:160-175 (1989).
Smith et al., Virology 123:2393-2406 (1982).
Journal of Virology vol. 54, No. 2, May 1985, pp. 436-445, Baltimore, U.S.; K. Iatrou et al.: "Polyhedrin Gene of Bombyx mori Nuclear Polyhedrosis Virus".
Virology vol. 131, No. 2, Dec. 1983, pp. 561-565, N.Y., U.S.; B. J. L. Hooft Van Iddekinge et al.: "Nucleotide Sequence of the Polyhedrin Gene of Autographa californica Nuclear Polyhedrosis Virus".
Chemical Abstracts vol. 99, No. 19, Nov. 7, 1983, p. 314, abstract No. 154864r, Columbus, Ohio, U.S.; M. V. Ashok et al.: "Centain observations on the physicochemical properties of nuclear polyhedrosis virus of Spodoptera litura (Fabricius)".
J. Entomol. Res. 1981, vol. 5, No. 1, pp. 43-46.
Chemical Abstracts vol. 83, No. 15, Oct. 13, 1975, p. 249, Abstract No. 128489x, Columbus, Ohio, U.S.; V. M. Pawar et al.: "Amino acid and nucleic acid composition of the polyhedral inclusion bodies of the nuclear polyhedrosis virus of Spodoptera litura".
Curr. Sci. 1975, vol. 44, No. 13, pp. 475, 476.
J. Gen. Virology vol. 67, 1986, pp. 1073-1079, Colchester, GB; D. Leisy et al.: "Location and Nucleotide Sequence of the Orgyia pseudotsugata Single Nucleocapsid Nuclear Polyedrosis Virsu Polyhedrin Gene".
Maniatis et al., Molecular Cloning Laboratory Manual, Cold Spring Laboratory, pp. 10-15 (1982).

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