Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-07-21
2000-11-21
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
422 50, 422 681, 435 71, 736501, C12Q 168
Patent
active
061501034
ABSTRACT:
An array of biomolecules is formed from a solid substrate comprising a surface, where said surface is at least partially covered with a layer of poly(ethylenimine) (PEI), and the layer is divided among a plurality of discrete first regions abutted and surrounded by a contiguous second region. The first regions are defined by the presence of a biomolecule and PEI. The second region is defined by the presence of PEI and the substantial absence of the biomolecule. The array may be prepared by a process including the steps of providing a solid substrate having a surface, wherein a layer of poly(ethylenimine) (PEI) covers at least a portion of the surface. The layer contains a plurality of discrete first regions abutted and surrounded by a contiguous second region. The process includes the step of depositing a biomolecule into the first regions while maintaining the second region substantially free of the biomolecule.
REFERENCES:
patent: 4753983 (1988-06-01), Ngo
patent: 5250613 (1993-10-01), Bergstrom et al.
patent: 5756050 (1998-05-01), Ershow et al.
hhtp://chroma.mbt.washington.edu/mod.sub.-- www/array.html, Hamilton MicroLab Arrayer, modular print head, Nov. 19, 1998 and hhtp://chroma.mbt.washington.edu/mod.sub.-- www/hamilton.html, Modular Array Head, dated Mar. 17, 1998.
Schummer et al., "Inexpensive Handheld Device for the Construction of High-Density Nucleic Acid Arrays," BioTechniques 23: 1087-1092, 1997.
Alpert and Regnier, "Preparation Of A Porous Microparticulate Anion-Exchange Chromotography Support For Proteins," Journal of Chromatography 185: 375-392, 1979.
Blanchard et al., "High-density oligonucleotide arrays," Biosensors & Bioelectronics 11(6/7): 687-690, 1996.
Boussif et al., "A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine," Proc. Natl. Acad. Sci. USA 92: 7297-7301, 1995.
Bruil et al., "Poly(ethyleneimine) modified filters for the removal of leukocytes from blood," Journal of Biomedical Materials Research 27: 1253-1268, 1993.
Chetverin and Kramer, "Oligonucleotide Arrays: New Concepts and Possibilities," Bio/Technology 12: 1093-1099, 1994.
D'Souza et al., "Immobilization of Yeast Cells By Adhesion To Glass Surface Using Polyethylenimine," Biotechnology Letters 8(9): 643-648, 1986.
Eggers et al., "Genosensors: microfabricated devices for automated DNA sequence analysis," SPIE Advances in DNA Sequences Technology 189: 113-126, 1993.
Ghosh and Musso, "Covalent attachment of oligonucleotides to solid supports," Nucleic Acids Research 15(13): 5353-5372, 1987.
Kremsky et al., "Immobilization of DNA via oligonucleotides containing an aldyhyde or carboxylic acid group at the 5' terminus," Nucleic Acids Research 15(7): 2891-2909, 1987.
Lipshutz et al., "Using Oligonucleotide Probe Arrays To Access Genetic Diversity," BioTechniques 19(3): 442-447, 1995.
O'Donnell-Maloney and Little, "Microfabrication and array technologies for DNA sequencing and diagnostics," Genetic Analysis: Biomolecular Engineering 13: 151-157, 1996.
Povey et al., "Trapping of Chemical Carcinogens with Magnetic Polyethyleneimine Microcapsules: I. Microcapsule Preparation and In Vitro Reactivity of Encapsulated Nucleophiles," Journal of Pharmaceutical Sciences 75(9): 831-837, 1986.
Povey et al., "Trapping of Chemical Carcinogens with Magnetic Polyethyleneimine Microcapsules: III. In Vivo Trapping of Electrophiles from N-Methyl-N-nitrosourea and Recovery from Feces," Journal of Pharmaceuticals Sciences 76(3): 201-207, 1987.
Rassi and Horvath, "High-Performance Liquid Chromatography of Nucleic Acid Constituents: Chromatographic Examination of Novel Stationary Phases," Chromatographia 19: 9-18, 1984.
Rounds et al., "Factors Contributing To Intrinsic Loading Capacity In Silica-Based Packing Materials For Preparative Anion-Exchange Protein Chromatography," Journal of Chromatography 362: 187-196, 1986.
Schena, "Genome analysis with gene expression microarrays," BioEssays 18(5): 427-431, 1996.
Schurer et al., "Polyethyleneimine As Tracer Particle For (Immuno) Electron Microscopy," The Journal Of Histochemistry And Cytochemistry 25(5): 384-387, 1977.
Southern et al., "Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids," Nucleic Acids Research 22(8): 1368-1373, 1994.
Stipp, "The Idol of the Geeks," Fortune 135(40): p. 40, 1997.
Teletzke et al., "How Liquids Spread On Solids," Chem. Eng. Comm. 55: 41-81, 1987.
Trinh and Schnabel, "Ionic strength dependence of the stability of polyelectrolyte complexes," Die Angewandte Makromolekulare Chemie 212: 167-179, 1993.
Van Nessds and Chen, "The use of oligodeoxynucleotide probes in chaotrope-based hybridization solutions," Nucleic Acids Research 19(19): 5143-5151, 1991.
Van Ness et al., "A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays," Nucleic Acids Research 19(12): 3345-3350, 1991.
Vanecek and Regnier, "Macroporous High-Performance Anion-Exchange Supports for Proteins," Analytical Biochemistry 121: 156-169, 1982.
Wasserman and Hultin, "High-Yield Method for Immobilization of Enzymes," Biotechnology and Bioengineering XXII(2): 271-287, 1980.
Moynihan Kristen
Ness Jeffrey Van
Tabone John C.
Marschel Ardin H.
QIAGEN Genomics, Inc.
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