Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1998-03-30
1999-10-19
Achutamurthy, Ponnathapura
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435232, 4353201, 435874, 435135, 435141, 435142, 536 232, 536 237, 530350, 530300, C12N 120, C12N 988, C12N 1500, C07H 2104
Patent
active
059688050
ABSTRACT:
The present invention relates to a polypeptide comprising the amino acid sequence of SEQ ID NO:1 or a sequence of SEQ ID NO:1 where in, one or more amino acids are deleted, replaced or added, and the polypeptide having polyester synthase activity. A polyester synthase gene comprising DNA coding for the above polypeptide; a recombinant vector comprising the gene; and a transformant transformed with the recombinant vector is also provided.
REFERENCES:
Timm, A. and Steinbuchel, A., "Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1" European Journal of Biochemistry, vol. 209n No. 1, Oct. 1992, pp. 15-30.
Huisman, G.W. et al., "Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans " The Journal of Biological Chemistry, vol. 266, No. 4, Feb. 5, 1991, pp. 2191-2198.
Timm, A. et al., "A general method for identification of polyhydroxyalkanoic acid synthase genes from pseudomonads belonging to the rRNA group I" Applied Microbiology and Biotechnology, vol. 40, No. 5, Jan. 1994, pp. 669-670.
Steinbuchel, A. et al., "Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria" Fems Microbiology Reviews, vol. 103, No. 2-4, Dec. 1992, pp. 219-224.
Matsuzaki et al., Cloning of 2 polyester synthase genes possessed by Pseudomonas sp. 61-3,: Nippon Nogeikagaku Kaishi, vol. 71, Mar. 1997, p. 334.
Doi Yoshiharu
Fukui Toshiaki
Matsusaki Hiromi
Achutamurthy Ponnathapura
Japan Science and Technology Corporation
Saidha Tekchand
The Institute of Physical and Chemical Research
Wetherell, Jr. John R.
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