Polyelectrolyte treated glass for enzyme immobilization and prot

Details Polyelectrolyte treated glass for enzyme immobilization and prot Polyelectrolyte treated glass for enzyme immobilization and prot

1997-08-22

1999-07-13

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 23, 435174, 435183, 501 11, 501 53, C12Q 100, C12Q 137, C12N 1100, C12N 900

Patent

active

059225319

ABSTRACT:
Materials and methods for selectively adsorbing proteins are disclosed. Under the invention, controlled pore glass is treated with a polyelectrolyte layer which comprises at least one polymer having a cationic portion which is electrostatically attracted to the porous outer surface of the glass. The constituents of the polyelectrolyte are preferably selected to have an affinity for the predetermined protein to facilitate adsorption thereof on the polyelectrolyte treated glass. Advantageously, the materials of the present invention can be utilized to immobilize enzymes and also to separate proteins from other components in a medium.

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Wilson et al., American Chemical Society pp.1-51 (1990).
Braude et al; Biochimica et Biophysica Acta, vol. 580, pp. 15-23 (1979). Month Not Available. Please Print.

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